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PCR Master Mixes (High Fidelity)
Q5 Hot Start High-Fidelity 2X Master Mix
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Q5 Hot Start High-Fidelity 2X Master Mix
Q5 Hot Start High-Fidelity 2X Master Mix from New England Biolabs
(3)
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Citations:
(246)
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Supplier Page from
New England Biolabs for
Q5 Hot Start High-Fidelity 2X Master Mix
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Q5 Hot Start High-Fidelity 2X Master Mix
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0494S
Quantity
100 reactions
Applications
DNA Analysis
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Master Mix
Temperature
-20 C
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (246)
(1)
The Identification of Host Proteins That Interact with Non-Structural Proteins-1α and -1β of Porcine Reproductive and Respiratory Syndrome Virus-1
Viruses
December 16, 2023
Sofia Riccio, Kay Childs, Ben Jackson, Simon P. Graham, Julian Seago
cloning
, either
GoTaq® Long PCR Master Mix
(#
M4021
,
Promega
) or
Q5® High-Fidelity DNA Polymerase
(#
M0494S
,
NEB
) were used. 2.5.
DNA Purification
PCR
products, plasmids, and restriction fragments were purified
(2)
Leukoencephalopathy with Brain stem and Spinal cord involvement and Lactate elevation (LBSL): Report of a new family and a novel DARS2 mutation
Mol Genet Metab Rep
December 2, 2023
Wei-Lin Huang, Maija R. Steenari, Rebekah Barrick, Mariella T. Simon, Richard Chang, Shaya S. Eftekharian, Alexander Stover, Philip H. Schwartz, Alexandra Latini, Jose E. Abdenur
was analyzed by
PCR
using the Q5® Hot Start High-Fidelity 2× Master Mix (
New England BioLabs
, Cat.
M0494S
, Ipswich, USA), and the forward and reverse primers 5′- GAGGAGAATTCCAGAATTCAGTAG-3′ and 5′- CTGTTGGCATTTTTGGATTCTC-3′,
(3)
Differentiated cultures of an immortalized human neural progenitor cell line do not replicate prions despite PrP(C) overexpression
Prion
December 1, 2023
Slota JA, Wang X, Lusansky D, Medina SJ, Booth SA.
50 ng of genomic DNA were amplified with the Q5 Hot Start High-Fidelity 2× PCR Master Mix (cat no.
M0494L
,
NEB
) using primers 5’-TCTTTGTGACTATGTGGACTG-3’ and 5’-TGCCACATGCTTGAGGTTGGTT-3’. The
PCR
products were
(4)
PRR14 organizes H3K9me3-modified heterochromatin at the nuclear lamina
Nucleus (Austin, Tex.)
December 1, 2023
Kiseleva AA, Cheng YC, Smith CL, Katz RA, Poleshko A.
pCMV6-AN-mGFP-
PRR14
full-length vectors using Q5 Hot-start High-Fidelity Polymerase 2x Mix (
New England Biolabs
, cat#
M0494S
) and the following primers containing
HindIII
and
RsrII
restriction sites (Forward (
HindIII
): 5’- GCTTCTCAAGCTTGTACCATCCATGGACTTGCCCGG-3’;
(5)
ELOA3 : A primate-specific RNA polymerase II elongation factor encoded by a tandem repeat gene cluster
Science Advances
November 24, 2023
Marc A. J. Morgan, Saeid Mohammad Parast, Marta Iwanaszko, Yuki Aoi, DongAhn Yoo, Zachary J. Dumar, Benjamin C. Howard, Kathryn A. Helmin, Qianli Liu, William R. Thakur,
et al.
Q5 Hot Start High-Fidelity 2X Master Mix
according to the manufacturer’s protocol (
New England Biolabs
,
M0494L
).
Polymerase chain reaction (PCR
) cycling conditions were as follows: initial denaturation: 98°C (30
(6)
Shiftless Is a Novel Member of the Ribosome Stress Surveillance Machinery That Has Evolved to Play a Role in Innate Immunity and Cancer Surveillance
Viruses
November 23, 2023
Jamie A. Kelly, Jonathan D. Dinman
using 1 µL of cDNA, and 500 nM primers in a final volume of 35 µL using Q5 Hotstart Mastermix (
NEB
M0494S
). Primers for this
assay
are listed in Table 3.
PCR
conditions were: 98 °C for 30 s followed by 40 cycles
(7)
A novel pathway of levodopa metabolism by commensal Bifidobacteria
Scientific Reports
November 6, 2023
M. S. Cirstea, A. Creus-Cuadros, C. Lo, A. C. Yu, A. Serapio-Palacios, S. Neilson, S. Appel-Cresswell, B. B. Finlay
into a strip tube containing a pre-made 25 µL PCR mix (
Q5 Hot Start High-Fidelity 2X Master Mix
,
NEB
M0494L
) and 8F/926R 16S primers (F: AGAGTTTGATCCTGGCTCAG, R: CCGTCAATTCCTTTRAGTTT). The newly streaked plates
(8)
The Impact of p70S6 Kinase-Dependent Phosphorylation of Gemin2 in UsnRNP Biogenesis
International journal of molecular sciences
October 25, 2023
Esser LM, Li Q, Jüdt M, Kähne T, Stork B, Grimmler M, Wesselborg S, Peter C.
all
cloning
procedures
, the Q5 High-Fidelity 2× Master Mix (New ENGLAND BioLabs, Frankfurt, Germany, #
M0494S
) and
KLD Enzyme Mix
(
New ENGLAND BioLabs
, Frankfurt, Germany, #
M0554S
) were used. For
cloning
of the
(9)
Utilizing AAV-mediated LEAPER 2.0 for programmable RNA editing in non-human primates and nonsense mutation correction in humanized Hurler syndrome mice
Genome Biology
October 23, 2023
Zongyi Yi, Yanxia Zhao, Zexuan Yi, Yongjian Zhang, Gangbin Tang, Xiaoxue Zhang, Huixian Tang, Wei Zhang, Ying Zhao, Huayuan Xu,
et al.
for 1 h according to the manufacturer’s instructions. The targeted locus was
PCR
amplified with Q5 (
M0494L
,
NEB
) and the corresponding primers are listed in Additional file 3: Table S2.
PCR
products were shipped
(10)
A Baculovirus Expression Vector Derived Entirely from Non-Templated, Chemically Synthesized DNA Parts
Viruses
September 23, 2023
Christopher Nguyen, Amanda Ibe-Enwo, Jeffrey Slack
Polymerase Chain Reaction
All
polymerase chain reactions
(PCRs) were performed using Q5 2X Hot Start Mastermix (#
M0494
,
New England Biolabs
, Ipswich, MA, USA) according to the manufacturer’s instructions. Primers were used
(11)
tgCRISPRi: efficient gene knock-down using truncated gRNAs and catalytically active Cas9
Nature Communications
September 11, 2023
Ankush Auradkar, Annabel Guichard, Saluja Kaduwal, Marketta Sneider, Ethan Bier
sites were amplified with the help of specific primers and using the Q5 Hot-start master mix (
NEB
#
M0494S
).
PCR
products were purified prior to
Sanger sequencing
. Data were analyzed using an ICE tool by Synthego
(12)
Staphylococcus epidermidis activates keratinocyte cytokine expression and promotes skin inflammation through the production of phenol-soluble modulins
Cell Reports
September 1, 2023
Michael R. Williams, Michelle D. Bagood, Timothy J. Enroth, Zoie L. Bunch, Nina Jiang, Edward Liu, Samia Almoughrabie, Shadi Khalil, Fengwu Li, Samantha Brinton,
et al.
distilled water,Invitrogen,Cat# 10977-015 Q5 Hot Start High Fidelity Polymerase Master Mix (2×),
NEB
,Cat#
M0494L
Ultrapure Agarose
,
Thermofisher Scientific
,Cat# 16500500 1kb Plus DNA Ladder,
NEB
,Cat#
N3200L
Sybr Safe DNA Gel Stain
(13)
Deep screening of proximal and distal splicing-regulatory elements in a native sequence context
bioRxiv
August 25, 2023
Yocelyn Recinos, Dmytro Ustianenko, Yow-Tyng Yeh, Xiaojian Wang, Martin Jacko, Lekha V. Yesantharao, Qiyang Wu, Chaolin Zhang
using primers compatible with
Illumina sequencing
and NEB Q5 Hot Start High-Fidelity master mix (
NEB
M0494
) (see primers listed in Supplemental Table 1). After using QIAquick
PCR purification
for
clean-up
, samples
(14)
PCR cloning Intermediated Gibson assembly (PIG) for Constructing DNA Repair Templates in CRISPR-Cas9 Based Gene Editing
MicroPubl Biol
August 18, 2023
Dongsheng Han, Scott Churcher, Jared T. Nordman
primer,TCAGGGTTATTGTCTCATGAGCG pScarlessHD-sfGFP-DsRed,Addgene #80811
NEB
Q5® High-Fidelity 2X Master Mix
,
M0494
NEB® PCR Cloning Kit
,
E1202S
NEB
Gibson Assembly® Master Mix
,
E2611S
SYBR™ Safe DNA gel stain,S33102 NEB®
(15)
Two-step CRISPR-Cas9 protocol for transposable element deletion in D. melanogaster natural populations
STAR Protoc
August 15, 2023
Miriam Merenciano, Laura Aguilera, Josefa González
Propionic acid
,
Merck
,Cat # 81910-1L Nipagin,Merck,Cat # H5501-500GR Q5 Hot Start High-Fidelity 2× Master Mix,
NEB
,Cat #
M0494S
DreamTaq Green PCR Master Mix (2×),
Thermo Fisher
,Cat #
K1081
,, Critical commercial
assays
,Critical
(16)
Targeting Sex Determination to Suppress Mosquito Populations
Research Square
July 27, 2023
Omar Akbari, Ming Li, Nikolay Kandul, Ruichen Sun, Ting Yang, Elena Dalla Dalla Benetta, Daniel Brogan, igor antoshechkin, Héctor Sánchez Sánchez C, Yin Peng Zhan,
et al.
(Integrated DNA Technologies) using Q5 Hotstart Start High-Fidelity 2X Master Mix (
New England Biolabs
, Cat. #
M0494S
). The generated plasmids were transformed into Zymo JM109 chemically competent
E. coli
(
Zymo Research
,
(17)
The contributions of DNA accessibility and transcription factor occupancy to enhancer activity during cellular differentiation
bioRxiv
July 27, 2023
Trevor Long, Tapas Bhattacharyya, Andrea Repele, Madison Naylor, Sunil Nooti, Shawn Krueger, Manu
and the longer amplicon. The in/out PCRs were carried out with Q5 polymerase (
New England BioLabs
,
M0494S
), while the out/out PCRs were carried out with
PrimeSTAR GXL DNA Polymerase
(
Takara Bio
,
R051A
). Seamless
(18)
Regulation of store-operated Ca2+ entry by IP3 receptors independent of their ability to release Ca2+
eLife
July 19, 2023
Pragnya Chakraborty, Bipan Kumar Deb, Vikas Arige, Thasneem Musthafa, Sundeep Malik, David I Yule, Colin W Taylor, Gaiti Hasan
modifications were carried out using Q5 Hot Start high-fidelity 2 X Master Mix (
New England BioLabs
, Cat#
M0494L
) using the recommendations of the manufacturer. Primers used in this study (details given in Supplementary
(19)
SPIDR: a highly multiplexed method for mapping RNA-protein interactions uncovers a potential mechanism for selective translational suppression upon cellular stress
bioRxiv
July 17, 2023
Erica Wolin, Jimmy K. Guo, Mario R. Blanco, Andrew A. Perez, Isabel N. Goronzy, Ahmed A. Abdou, Darvesh Gorhe, Mitchell Guttman, Marko Jovanovic
separate tube. Biotinylated oligo tags were amplified on-bead using 2X Q5 Hot-Start Mastermix (
NEB
#
M0494
) with primers that add the indexed full Illumina adaptor sequences. To isolate barcoded cDNA, the supernatant
(20)
Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathy
Nature Communications
June 22, 2023
Markus Grosch, Laura Schraft, Adrian Chan, Leonie Küchenhoff, Kleopatra Rapti, Anne-Maud Ferreira, Julia Kornienko, Shengdi Li, Michael H. Radke, Chiara Krämer,
et al.
Nextera-compatible adapters (Supplementary Data 6) using Q5® Hot Start High-Fidelity 2× Master Mix (
M0494L
,
NEB
). One microliter of a
1:100
dilution was used for a second
PCR
attaching sample-specific index
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Reviews
Write a Review
(1)
10/6/2023
Convenient PCR Master Mix From New England Biolabs
Anh Nguyen
I used this Master Mix for running PCR which contained all the enzymes and reagents needed. I only needed to add primers, a DNA template, and water to this Master Mix for PCR reactions.
(1)
3/31/2023
Good PCR Master Mix
YONG M
Try this master mix for hard PCR application from genomic DNA. It's simple to use and the mix includes every component. The enzyme has high fidelity and could amplify the difficult fragment.
(1)
1/2/2020
DNA Insert Amplification for Cloning
Dr. Thippeswamy Sannasiddappa
Highly recommended for amplifying error free longer length DNA inserts. Also, reactions can be set up room temperature.
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Q5 Hot Start High-Fidelity 2X Master Mix from New England Biolabs
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