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PCR Master Mixes (High Fidelity)
Q5 Hot Start High-Fidelity 2X Master Mix
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Q5 Hot Start High-Fidelity 2X Master Mix
Q5 Hot Start High-Fidelity 2X Master Mix from New England Biolabs
(3)
Write a review
Citations:
(255)
Supplier Page
Supplier Page from
New England Biolabs for
Q5 Hot Start High-Fidelity 2X Master Mix
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Q5 Hot Start High-Fidelity 2X Master Mix
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0494S
Quantity
100 reactions
Applications
DNA Analysis
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Master Mix
Temperature
-20 C
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (255)
(101)
Viperin triggers ribosome collision-dependent translation inhibition to restrict viral replication
Molecular cell
May 5, 2022
Hsu JC, Laurent-Rolle M, Pawlak JB, Xia H, Kunte A, Hee JS, Lim J, Harris LD, Wood JM, Evans GB,
et al
.
Gibson Assembly Master Mix
,
New England Biolabs
,Cat#
E2611
Q5 Hot Start High-Fidelity 2X Master Mix
,
New England Biolabs
,Cat#
M0494
ZymoPURE II Plasmid Midiprep Kit,
Zymo Research
,Cat#
D4201
MEGAscript T7 Transcription Kit
,
Thermo Fisher Scientific
(102)
Functional and Structural Characterization of Diverse NfsB Chloramphenicol Reductase Enzymes from Human Pathogens
Microbiology Spectrum
April 27, 2022
Michael W. Mullowney, Natalia I. Maltseva, Michael Endres, Youngchang Kim, Andrzej Joachimiak, Terence S. Crofts
FA1090, gi: AAW89132). Each ORF was amplified by
PCR
by Q5 high fidelity polymerase (
New England Biolabs
,
M0494S
) according to manufacturer recommendations with primers that contained 25-bp homology to pZE21-derived
(103)
Rapid and Accurate Detection of SARS Coronavirus 2 by Nanopore Amplicon Sequencing
Frontiers in Microbiology
April 6, 2022
Xiao-xiao Li et al.
targeted
amplification
of the
SARS-CoV-2
genome with the
Q5 Hot-Start High-Fidelity 2x Master Mix
(
M0494
;
NEB
, Ipswich, MA, United States) and primer pools A and B (
PCR
tiling of
COVID-19 virus
, version 4;
(104)
A P53-Independent DNA Damage Response Suppresses Oncogenic Proliferation and Genome Instability
SSRN Electronic Journal
April 4, 2022
Katerina Fagan-Solis et al.
Kit
QIAGEN
74136
Comet Assay Kit Trevigen 4250-050-K
Q5 Hot Start High-Fidelity 2X Master Mix
New England Biolabs
M0494S
NEBuilder HiFi DNA Assembly Master Mix
New England Biolabs
E2621L
EdU-Click 594 Baseclick BCK-Edu594 TOPO
(105)
Epigenetic inheritance is gated by naïve pluripotency and Dppa2
The EMBO journal
April 4, 2022
Carlini V, Policarpi C, Hackett JA.
microlitre of this product was used for
PCR amplification
using Q5 hot start high‐fidelity polymerase (
NEB
#
M0494S
) and a unique combination of the dual‐barcoded primers P5 and P7 Nextera XT Index kit (Illumina #15055293)
(106)
Inducible CRISPR activation screen for interferon-stimulated genes identifies OAS1 as a SARS-CoV-2 restriction factor
PLoS pathogens
April 1, 2022
Danziger O, Patel RS, DeGrace EJ, Rosen MR, Rosenberg BR.
100ng of each gDNA sample was
PCR
amplified in triplicate with
Q5 High-Fidelity DNA Polymerase
(
NEB
#
M0494S
) with 500nM primers (S4 Table) flanking the guide sequence cassette and including Illumina adaptor sequences
(107)
A High-Efficiency AAV for Endothelial Cell Transduction Throughout the Central Nervous System
Nat Cardiovasc Res
April 1, 2022
Krolak T, Chan KY, Kaplan L, Huang Q, Wu J, Zheng Q, Kozareva V, Beddow T, Tobey IG, Pacouret S,
et al
.
to
PCR
amplify a modified AAV9 template (K449R) using
Q5 Hot Start High-Fidelity 2X Master Mix
(
NEB
,
M0494S
) following the manufacturer’s protocol. The
PCR
products were cleaned up using AMPure XP beads (
Beckman
,
(108)
Clamping, Bending, and Twisting Inter-Domain Motions in the Misfold-Recognising Portion of UDP-Glucose: Glycoprotein Glucosyl-Transferase
SSRN Electronic Journal
March 30, 2022
Carlos P. Modenutti et al.
peptides, and recombinant proteins
Q5 Hot Start High-Fidelity 2X Master Mix
New England Biolabs
Cat#
M0494S
NEBuilder HiFi DNA Assembly Master Mix
New England Biolabs
Cat#
E2621S
Gibson Assembly Kit
New England Biolabs
(109)
Two-Component Spike Nanoparticle Vaccine Protects Macaques from SARS-CoV-2 Infection
SSRN Electronic Journal
March 30, 2022
Philip J. M. Brouwer et al.
assays
SSIV Reverse Transcriptase
Thermo Fisher
Cat#
18090050
Q5 Hot Start DNA Polymerase
NEB
Cat#
M0494
Ligation
Sequencing
Kit Nanopore Cat# SQK-LSK109
Nano-Glo Luciferase Assay
System
Promega
Cat#
(110)
DNA spike-ins enable confident interpretation of SARS-CoV-2 genomic data from amplicon-based sequencing
bioRxiv
March 29, 2022
Kim A. Lagerborg et al.
enzyme and corresponding buffer. We also tested
NEB
Q5 Hot Start High-fidelity 2x Master Mix
(
NEB
#
M0494L
), NEB
Q5 Hot Start High-fidelity 2x Master Mix
plus .01% SDS, NEB Q5 Ultra II Master Mix (
NEB
#
M0544L
),
(111)
Serine/Threonine Protein Phosphatase 2A Regulates the Transport of Axonal Mitochondria
Frontiers in Cellular Neuroscience
March 18, 2022
Keunjung Heo, Himanish Basu, Amos Gutnick, Wei Wei, Evgeny Shlevkov, Thomas L. Schwarz
was generated using Q5 Hot Start High-Fidelity
site-directed mutagenesis
kit (
New England Biolabs
, #
M0494
, Ipswich, MA, United States). mCherry-Rab7 (#55127) and mEGFP-N1 (#54767) were obtained from Addgene.
Cell Culture
(112)
Transgenic
Drosophila
lines for LexA-dependent gene and growth regulation
G3 Genes|Genomes|Genetics
March 4, 2022
Kathleen R Chang et al.
of PCR master mix containing 7 μl of water, 10 μl of Q5 Hot Start High-Fidelity 2× Master Mix (
NEB
M0494S
), 1 μl of 10 μM shRNA_GA_F primer (5′-GAGAACTCTGAATAGATCTGTTCTAGAAAACATCCCATAAAACATCCCATATTCA-3′), and
(113)
Engineering Novel Lentiviral Vectors for Labelling Tumour Cells and Oncogenic Proteins
Bioengineering (Basel, Switzerland)
February 25, 2022
Akgül S, Offenhäuser C, Kordowski A, Day BW.
632443)
Q5 Hot Start High-Fidelity 2X Master Mix
(
New England BioLabs
, Notting Hill, VIC, Australia, cat.
M0494S
)
XhoI
restriction enzyme (
New England BioLabs
, cat.
R0146S
)
EcoRI-HF
restriction enzyme (
New England BioLabs
,
(114)
Optimization of the SARS-CoV-2 ARTIC Network V4 Primers and Whole Genome Sequencing Protocol
Frontiers in Medicine
February 17, 2022
Arnold W. Lambisia et al.
by combining 3μl of nuclease-free water, 6.25 μl of
Q5® Hot Start High-Fidelity 2X Master Mix
(
NEB
M0494
, MA, USA), 2 μl of primer pool and 1.3 μl of cDNA. The V4 primer pools
amplification
employed two reactions
(115)
Immunization with synthetic SARS-CoV-2 S glycoprotein virus-like particles protects macaques from infection
Cell Reports Medicine
February 15, 2022
Guidenn Sulbaran et al.
,
Thermo Fisher scientific
,Cat#
18090050
Ligation
Sequencing
Kit,Nanopore,Cat# SQK-LSK109 Q5 Hot Start DNA Polymerase,
NEB
,Cat#
M0494
Expi293™ Expression System Kit
,
Thermo Fisher scientific
,Cat#
A14635
Nano-Glo Luciferase Assay
System,
Promega
,Cat#
(116)
Transmission from vaccinated individuals in a large SARS-CoV-2 Delta variant outbreak
Cell
February 3, 2022
Katherine J. Siddle, Lydia A. Krasilnikova, Gage K. Moreno, Stephen F. Schaffner, Johanna Vostok, Nicholas A. Fitzgerald, Jacob E. Lemieux, Nikolaos Barkas, Christine Loreth, Ivan Specht,
et al.
#
S1330S
AMPure XP,
Beckman Coulter
,Cat #
A63881
Q5 Hot Start High-Fidelity 2X Master Mix
,
New England BioLabs
,Cat #
M0494S
Illumina DNA Prep,Illumina,Cat #20018705 IDT for Illumina DNA/RNA UD Indexes Set A-D,Illumina,Cat
(117)
Identification of high-confidence human poly(A) RNA isoform scaffolds using nanopore sequencing
RNA
February 1, 2022
Logan Mulroney et al.
of the TSO) and a gene-specific reverse primer with Q5 Hot Start High-Fidelity 2× Master Mix (
NEB
, #
M0494S
). cDNA was prepared for
sequencing
using the barcoded NBD 104 expansion of the SQK-LSK109 protocol following
(118)
Monoclonal antibodies targeting two immunodominant epitopes on the Spike protein neutralize emerging SARS-CoV-2 variants of concern
eBioMedicine
February 1, 2022
Branislav Kovacech et al.
even-numbered tiled amplicons. The
PCR
was performed using a
Q5 Hot Start High-Fidelity 2X Master Mix
(
M0494
,
New England Biolabs
) and the cycling conditions were: 30 sec at 98°C (initial denaturation), followed
(119)
Protein visualization and manipulation in Drosophila through the use of epitope tags recognized by nanobodies
eLife
January 25, 2022
Xu J, Kim AR, Cheloha RW, Fischer FA, Li JSS, Feng Y, Stoneburner E, Binari R, Mohr SE, Zirin J,
et al
.
fragments were amplified using Phusion polymerase (New England Biolabs [NEB], M0530) or Q5 polymerase (
NEB
,
M0494
). Linearized plasmids and
PCR
fragments were gel purified using QIAquick columns (
Qiagen
, #
28115
) and
(120)
Single-cell delineation of lineage and genetic identity in the mouse brain
Nature
January 20, 2022
Rachel C. Bandler et al.
site.
Ligation
products were amplified by
PCR
using
Q5 Hot Start High Fidelity 2x Master Mix
(
NEB
, #
M0494
) using primers targeting the STICR
sequencing
primer site and the adapter sequence using the following
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Reviews
Write a Review
(1)
10/6/2023
Convenient PCR Master Mix From New England Biolabs
Anh Nguyen
I used this Master Mix for running PCR which contained all the enzymes and reagents needed. I only needed to add primers, a DNA template, and water to this Master Mix for PCR reactions.
(1)
3/31/2023
Good PCR Master Mix
YONG M
Try this master mix for hard PCR application from genomic DNA. It's simple to use and the mix includes every component. The enzyme has high fidelity and could amplify the difficult fragment.
(1)
1/2/2020
DNA Insert Amplification for Cloning
Dr. Thippeswamy Sannasiddappa
Highly recommended for amplifying error free longer length DNA inserts. Also, reactions can be set up room temperature.
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Q5 Hot Start High-Fidelity 2X Master Mix from New England Biolabs
Supplier Page
View Company Product Page
Pricing Info
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