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PCR Master Mixes (High Fidelity)
Q5 Hot Start High-Fidelity 2X Master Mix
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Q5 Hot Start High-Fidelity 2X Master Mix
Q5 Hot Start High-Fidelity 2X Master Mix from New England Biolabs
(3)
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Citations:
(255)
Supplier Page
Supplier Page from
New England Biolabs for
Q5 Hot Start High-Fidelity 2X Master Mix
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Q5 Hot Start High-Fidelity 2X Master Mix
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0494S
Quantity
100 reactions
Applications
DNA Analysis
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Master Mix
Temperature
-20 C
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (255)
(81)
Optimized In Vitro CRISPR/Cas9 Gene Editing Tool in the West Nile Virus Mosquito Vector, Culex quinquefasciatus
Insects
September 19, 2022
Tran Zen B. Torres, Brian C. Prince, Alexis Robison, Claudia Rückert
Drosophila
-optimized plasmid. PCRs were carried out with Q5® Hot Start High-Fidelity Master Mix (
New England Biolabs
, #
M0494
). Multiple overlapping amplicons were assembled using
NEBuilder HiFi DNA Assembly Master Mix
(
New England Biolabs
(82)
Protocol for construction and characterization of direct electron transfer-based enzyme-electrode using gold binding peptide as molecular binder
STAR Protocols
September 16, 2022
Hyeryeong Lee, Eun Mi Lee, Stacy Simai Reginald, In Seop Chang
recombinant proteins" ,, Q5® Hot Start High-Fidelity 2× Master Mix,"New England Biolabs (Ipswich, MA, USA)",
M0494S
KLD enzyme mix
,"
New England Biolabs
(Ipswich, MA, USA)",
M0554S
Expin™
PCR
SV,"GeneAll (Seoul, South
(83)
Combinatorial optimization of mRNA structure, stability, and translation for RNA-based therapeutics
bioRxiv
September 5, 2022
Kathrin Leppek et al.
PCR
was performed under following conditions: 1 μL RT reaction, 10 μL 2x Q5 Hot Start Master Mix (
NEB
M0494S
), 0.2 μL 100x SYBR (
Thermo
S7563
), 1 μL 10uM Read1Partial_F, 1 μL 10 uM 50:50 Hbb_Fwd:Nluc_Fwd mix in
(84)
Dependence on a variable residue limits the breadth of an HIV MPER neutralizing antibody, despite convergent evolution with broadly neutralizing antibodies
PLOS Pathogens
September 2, 2022
Cathrine Scheepers et al.
(catalog #
210519
,
Agilent Technologies
, CA, USA) or through Gibson Assembly (NEB Q5 HS 2X MM catalog #
M0494S
;
NEB
CutSmart 10X Buffer catalog #
B7204S
,
NEB
DpnI
catalog #
R0176L
and
NEBuilder HiFi DNA Assembly Master Mix
(85)
Mutational dynamics across VOCs in International travellers and Community transmission underscores importance of Spike-ACE2 interaction☆
Microbiological Research
September 1, 2022
Priyanka Mehta, Varsha Ravi, Priti Devi, Ranjeet Maurya, Shaista Parveen, Pallavi Mishra, Aanchal Yadav, Aparna Swaminathan, Sheeba Saifi, Kriti Khare,
et al.
(IDT Product number: 10007184) and
Q5 ® High-Fidelity 2X master mix
(
New England Biolabs
, Cat. No.
M0494S
). For
sequencing
library preparation, the amplified products were ligated with rapid barcode sequences
(86)
SARS-CoV-2 VOCs, Mutational diversity and clinical outcome: Are they modulating drug efficacy by altered binding strength?
Genomics
September 1, 2022
Sheeba Saifi, Varsha Ravi, Sparsh Sharma, Aparna Swaminathan, Nar Singh Chauhan, Rajesh Pandey
primers (IDT Product number: 10007184) and Q5 High-Fidelity 2× master mix (
New England Biolabs
, Cat. No.
M0494S
). The amplicons were then ligated to the unique barcode sequences and purified using SPRI beads. The
(87)
Single-cell analysis of hepatoblastoma identifies tumor signatures that predict chemotherapy susceptibility using patient-specific tumor spheroids
Nature communications
August 25, 2022
Song H, Bucher S, Rosenberg K, Tsui M, Burhan D, Hoffman D, Cho SJ, Rangaswami A, Breese M, Leung S,
et al
.
by
polymerase chain reaction
using
Q5® Hot Start High-Fidelity 2X Master Mix
(
NEB
, Ipswich, Ma, Cat:
M0494S
), then sequenced by
Sanger sequencing
(MCLAB, South San Francisco, CA) using the same primers. Reporting
(88)
Detection of Microsatellite Instability in Colonoscopic Biopsies and Postal Urine Samples from Lynch Syndrome Cancer Patients Using a Multiplex PCR Assay
Cancers (Basel)
August 8, 2022
Rachel Phelps et al.
round of
PCR
, Q5 Hot Start High-Fidelity 2× Master Mix (
New England BioLabs
, Ipswich, MA, USA, cat. no.
M0494S
) was used in the second, and an Ampure bead
clean-up
was introduced between the first and second round
(89)
Direct epitranscriptomic regulation of mammalian translation initiation through N4-acetylcytidine
Molecular cell
August 4, 2022
Arango D, Sturgill D, Yang R, Kanai T, Bauer P, Roy J, Wang Z, Hosogane M, Schiffers S, Oberdoerffer S.
templates were generated by
high fidelity PCR
using
Q5 Hot Start High-Fidelity 2X Master Mix
(
NEB
, Cat. #:
M0494
), and a forward primer specific for the first 20 nt of the Firefly luciferase CDS with an overhang corresponding
(90)
PRD-2 mediates clock-regulated perinuclear localization of clock gene RNAs within the circadian cycle of Neurospora
Proceedings of the National Academy of Sciences of the United States of America
August 2, 2022
Bartholomai BM, Gladfelter AS, Loros JJ, Dunlap JC.
(pRS426) were amplified by
PCR
using
Q5 Hot Start High-Fidelity 2X Master Mix
(
New England Biolabs
,
M0494
). The fragments were assembled using
NEBuilder HIFI DNA Assembly Master Mix
(
New England Biolabs
,
E2621
).
(91)
Acylation probing of “generic” RNA libraries reveals critical influence of loop constraints on reactivity
Cell Chemical Biology
August 1, 2022
Lu Xiao, Linglan Fang, Eric T. Kool
mix,
New England BioLabs
,CAT#
M0531S
Q5 hot start high fidelity PCR master mix,
New England BioLabs
,CAT#
M0494S
HiScribe™ T7 Quick High Yield
RNA Synthesis
Kit,
New England BioLabs
,CAT#
E2050S
MiniElute gel extraction
(92)
Polyadenylation ligation‐mediated sequencing (PALM‐Seq) characterizes cell‐free coding and non‐coding RNAs in human biofluids
Clinical and Translational Medicine
July 20, 2022
Zhongzhen Liu et al.
5‐μl
PCR
primers (5‐μM each) and 25 μl of 2× Q5 Hot Start PCR Master Mix (
New England Biolabs
, cat.
M0494
) were added.
PCR
was carried out following the manufacturer's manual, with annealing temperature of
(93)
Sequencing during Times of Change: Evaluating SARS-CoV-2 Clinical Samples during the Transition from the Delta to Omicron Wave
Viruses
June 28, 2022
Shuchen Feng, Mudassir S. Ali, Monika Evdokimova, Gail E. Reid, Nina M. Clark, Susan L. Uprichard, Susan C. Baker
positive control in this experiment. A
PCR
reaction was performed using
Q5® High-Fidelity DNA polymerase
(
M0494S
,
NEB
, Ipswich, MA, USA). The 50 µL reaction was performed using 2 µL of cDNA, 2.5 µL of 10 µM primer
(94)
Genome-Wide CRISPR Screening Identifies DCK and CCNL1 as Genes That Contribute to Gemcitabine Resistance in Pancreatic Cancer
Cancers (Basel)
June 27, 2022
Hai Yang et al.
redundant reactions, each containing 5 μg DNA, 50 μL
Q5® Hot Start High-Fidelity 2X Master Mix
(
NEB
#
M0494L
), and 3 μL of a 10 μM solution of each universal illumine adapter tailed primer (P5 and P7). We structured
(95)
Body Composition and Metabolic Changes in a Lyon Hypertensive Congenic Rat and Identification of Ercc6l2 as a Positional Candidate Gene
Frontiers in Genetics
June 24, 2022
Karen C. Clark, Valerie A. Wagner, Katie L. Holl, John J. Reho, Monika Tutaj, Jennifer R. Smith, Melinda R. Dwinell, Justin L. Grobe, Anne E. Kwitek
spleen
gDNA, 500 ng primers (Supplementary Table S6), and
Q5 Hot-Start High-Fidelity 2X Master Mix
(Cat#
M0494S
,
New England Biolabs
) before verification by
Sanger sequencing
(Functional Biosciences, Inc., Madison,
(96)
Dynamic spreading of chromatin-mediated gene silencing and reactivation between neighboring genes in single cells
eLife
June 9, 2022
Lensch S, Herschl MH, Ludwig CH, Sinha J, Hinks MM, Mukund A, Fujimori T, Bintu L.
delta Ct to Beta-actin.
PCR
on cDNA was performed using Q5 Hot Start High-Fidelity 2 X Master Mix (
NEB
,
M0494L
) on a
C1000 Touch
Thermal Cycler from
Bio-Rad
.
PCR
products were run on a 1–1.5% TAE agarose gel at
(97)
SARS-CoV-2 Variants of Concern Hijack IFITM2 for Efficient Replication in Human Lung Cells
Journal of Virology
June 8, 2022
Rayhane Nchioua et al.
ARTIC nCoV-2019 V3 primer set (IDT) and the Q5 Hot Start high-fidelity 2× master mix (
NEB
, catalog no.
M0494S
) were used with modified manufacturer’s instructions. The primer pools 1 and 2 were diluted to a final
(98)
A Molecular Analysis of Memory B Cell and Antibody Responses Against Plasmodium falciparum Merozoite Surface Protein 1 in Children and Adults From Uganda
Frontiers in Immunology
June 2, 2022
S. Jake Gonzales et al.
the linear expression cassettes (2 µl at 1 ng/µl) using 10 µl
NEB
Q5 Hot Start HiFi PCR master mix (#
M0494S
), 6 µl nuclease-free water and 1 µl sequence-specific F and R primer (10 µM, f/c 500 nM) that were based
(99)
Reducing metabolic burden in the PACEmid evolver system by remastering high‐copy phagemid vectors
Engineering Biology
June 1, 2022
Beth India Davenport, Jure Tica, Mark Isalan
appropriate. 5.2
Molecular cloning
and plasmid sources Q5 Hot‐Start High‐Fidelity polymerase (
NEB
,
M0494S
) was used for DNA fragment
amplification
. All
PCR reactions
were undergone by following manufacturer
(100)
Harnessing stepping-stone hosts to engineer, select, and reboot synthetic bacteriophages in one pot
Cell Reports Methods
May 23, 2022
Li Cheng, Ziqing Deng, Haoran Tao, Wenchen Song, Bo Xing, Wenfeng Liu, Lingxin Kong, Shengjian Yuan, Yingfei Ma, Yayun Wu,
et al.
recombinant proteins","Chemicals, peptides, and recombinant proteins" ,,
Q5 High-Fidelity 2X Master Mix
,
NEB
,
M0494S
DNase I
,
SIGMA
,Cat #
DN25
RNaseA,
Invitrogen
,Cat #
12091021
Proteinase K
,
NEB
,
P8107S
BsaI-HFv2
,
NEB
,
R3733S
BbsI-HF
,
NEB
,
R3539S
T4 DNA ligase
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Reviews
Write a Review
(1)
10/6/2023
Convenient PCR Master Mix From New England Biolabs
Anh Nguyen
I used this Master Mix for running PCR which contained all the enzymes and reagents needed. I only needed to add primers, a DNA template, and water to this Master Mix for PCR reactions.
(1)
3/31/2023
Good PCR Master Mix
YONG M
Try this master mix for hard PCR application from genomic DNA. It's simple to use and the mix includes every component. The enzyme has high fidelity and could amplify the difficult fragment.
(1)
1/2/2020
DNA Insert Amplification for Cloning
Dr. Thippeswamy Sannasiddappa
Highly recommended for amplifying error free longer length DNA inserts. Also, reactions can be set up room temperature.
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Q5 Hot Start High-Fidelity 2X Master Mix from New England Biolabs
Supplier Page
View Company Product Page
Pricing Info
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Company Info
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