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Products
PCR Master Mixes (High Fidelity)
Q5 Hot Start High-Fidelity 2X Master Mix
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Q5 Hot Start High-Fidelity 2X Master Mix
Q5 Hot Start High-Fidelity 2X Master Mix from New England Biolabs
(3)
Write a review
Citations:
(253)
Supplier Page
Supplier Page from
New England Biolabs for
Q5 Hot Start High-Fidelity 2X Master Mix
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Q5 Hot Start High-Fidelity 2X Master Mix
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0494S
Quantity
100 reactions
Applications
DNA Analysis
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Master Mix
Temperature
-20 C
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (253)
(61)
Engineering artificial photosynthetic life-forms through endosymbiosis
Nature Communications
December 1, 2022
Jason E. Cournoyer, Sarah D. Altman, Yang-le Gao, Catherine L. Wallace, Dianwen Zhang, Guo-Hsuen Lo, Noah T. Haskin, Angad P. Mehta
Defined DNA fragments were amplified using
PCR
(
Q5 Hot Start High-Fidelity 2X Master mix
,
NEB
catalog #
M0494S
) and inserted into defined sites in the host vectors using Gibson assembly59. Double-stranded oligonucleotide
(62)
Rapid expansion and extinction of antibiotic resistance mutations during treatment of acute bacterial respiratory infections
Nature Communications
December 1, 2022
Hattie Chung et al.
mix was the following: 2 µL DNA template, 10 µL
Q5 Hot-Start High-Fidelity 2X Master Mix
, 1 µL (
NEB
#
M0494S
), 1 µL locus-specific forward primer with UMIs, 1 µL locus-specific reverse primer with UMIs (primers
(63)
Combinatorial optimization of mRNA structure, stability, and translation for RNA-based therapeutics
Nature Communications
December 1, 2022
Kathrin Leppek et al.
PCR
was performed under following conditions: 1 µL RT reaction, 10 µL 2x Q5 Hot Start Master Mix (
NEB
M0494S
), 0.2 µL 100x SYBR (
Thermo
S7563
), 1 µL 10 µM Read1Partial_F, 1 µL 10 µM 50:50 Hbb_Fwd:Nluc_Fwd mix
(64)
Characterization of Cme and Yme thermostable Cas12a orthologs
Communications Biology
December 1, 2022
Ryan T. Fuchs, Jennifer L. Curcuru, Megumu Mabuchi, Audrey Noireterre, Peter R. Weigele, Zhiyi Sun, G. Brett Robb
digestion reactions were either generated by
PCR
using Q5® High-Fidelity 2× Hot Start Master Mix (
NEB
#
M0494
) or oligonucleotides produced by IDT. PAM and cleavage site pattern determination using a small, circular
(65)
Multiplex base editing to convert TAG into TAA codons in the human genome
Nature Communications
December 1, 2022
Yuting Chen, Eriona Hysolli, Anlu Chen, Stephen Casper, Songlei Liu, Kevin Yang, Chenli Liu, George Church
C3040I
) was used following the manufacturer’s instructions.
Q5 High-Fidelity 2X Master Mix
(
NEB
cat#
M0494S
) was used for all PCRs. All enzymes and buffers were obtained from
New England Biolabs
unless otherwise
(66)
Harnessing DSB repair to promote efficient homology-dependent and -independent prime editing
Nature Communications
December 1, 2022
Martin Peterka et al.
instructions. For off-target analysis, amplicons were generated using
Q5 Hot Start High-Fidelity 2x Master Mix
(
M0494
,
NEB
). Amplicons for long-read
sequencing
were generated with Q5 High-Fidelity polymerase (
M0492S
,
NEB
)
(67)
Improving the specificity of nucleic acid detection with endonuclease-actuated degradation
Communications Biology
December 1, 2022
Roger S. Zou et al.
plasmid (Integrated DNA technologies 10006625), and 10 µL of Q5 2× master mix (
New England BioLabs
M0494
). Thermocycle at 98 °C for 30 s for initial denaturation, followed by 35 cycles of 98 °C for 10 s, 69
(68)
Induction of an immortalized songbird cell line allows for gene characterization and knockout by CRISPR-Cas9
Scientific Reports
December 1, 2022
Matthew T. Biegler, Olivier Fedrigo, Paul Collier, Jacquelyn Mountcastle, Bettina Haase, Hagen U. Tilgner, Erich D. Jarvis
Supplementary Table 9 for a list of primers) using
Q5 Hot Start High-fidelity 2X Master Mix
(
NEB
, Cat. #
M0494
). One exception was the CHD sextyping
amplification
protocol, which followed a
Taq polymerase
protocol
(69)
Systematic single-cell pathway analysis to characterize early T cell activation
Cell reports
November 22, 2022
Bibby JA, Agarwal D, Freiwald T, Kunz N, Merle NS, West EE, Singh P, Larochelle A, Chinian F, Mukherjee S,
et al
.
Microprep kit,
Zymo Research
,Cat#
D3021
Q5® Hot Start High-Fidelity 2X Master Mix
,
New England Biolabs
,Cat#
M0494
DNA Clean & ConcentratorTM,
Zymo Research
,Cat#
D4004
EasySep Human CD4+ T Cell Isolation Kit
,
Stemcell Technologies
(70)
PAC-FragmentDEL – photoactivated covalent capture of DNA-encoded fragments for hit discovery†
RSC Medicinal Chemistry
November 16, 2022
Huiyong Ma, James B. Murray, Huadong Luo, Xuemin Cheng, Qiuxia Chen, Chao Song, Cong Duan, Ping Tan, Lifang Zhang, Jian Liu,
et al.
PowerUp SYBR Green Master Mix
(
Thermo
,
A25778
) and amplified by
PCR
using
Q5 Hot Start High-Fidelity 2X Master Mix
(
NEB
,
M0494L
).
PCR
cycle was determined by
qPCR
result. The resulting
PCR
amplicons were purified by using QIAGEN-MinElute
(71)
Efficient Tracing of the SARS-CoV-2 Omicron Variants in Santa Barbara County Using a Rapid Quantitative Reverse Transcription PCR Assay
Diagnostics (Basel)
November 15, 2022
Zach Aralis, Stewart Comer, Henning Ansorg, Carl Palmer, Jennifer Smith, Stuart C. Feinstein, Lynn N. Fitzgibbons, Carolina Arias
ARTIC V3 Protocol [39]. The
PCR
reaction was prepared by mixing 5 µL of Q5 Hotstart 2X Master Mix [
NEB
,
M0494S
], 1.8µL of primer pool 1 or 2, 2.2 µL of nuclease-free water, and 1 µL of cDNA per sample.
Amplification
(72)
SARS-CoV-2 Variants of Concern and Variations within Their Genome Architecture: Does Nucleotide Distribution and Mutation Rate Alter the Functionality and Evolution of the Virus?
Viruses
November 11, 2022
Varsha Ravi, Aparna Swaminathan, Sunita Yadav, Hemant Arya, Rajesh Pandey
sets (IDT product number: 10007184) and
Q5® High-Fidelity 2X master mix
(
New England Biolabs
, Cat. No.
M0494S
). For
sequencing
library preparation, the amplified products were ligated with rapid barcode sequences
(73)
Multiphase coalescence mediates Hippo pathway activation
Cell
November 1, 2022
Li Wang, Kyungsuk Choi, Ting Su, Bing Li, Xiaofeng Wu, Ruihui Zhang, Jordan H. Driskill, Hongde Li, Huiyan Lei, Pengfei Guo,
et al.
assays
,Critical commercial
assays
Q5 Hot Start High-Fidelity 2× Master Mix,
New England BioLabs
,Cat#
M0494
NEBuilder HiFi DNA Assembly Master Mix
,
New England BioLabs
,Cat#
E2621
RNeasy Plus Mini Kit,
Qiagen
,Cat#
(74)
Transcription-coupled donor DNA expression increases homologous recombination for efficient genome editing
Nucleic Acids Research
October 28, 2022
Kaixuan Gao, Xuedi Zhang, Zhenwu Zhang, Xiangyu Wu, Yan Guo, Pengchong Fu, Angyang Sun, Ju Peng, Jie Zheng, Pengfei Yu,
et al.
from
human
embryonic kidney (HEK) 293T genomic DNA by using either
Q5 High-Fidelity DNA Polymerase
(
M0494S
,
New England Biolabs
) or KOD DNA Polymerase (KOD-101, Toyobo). HA inserts were cloned into KpnI and
(75)
Structural basis for Cas9 off-target activity
Cell
October 27, 2022
Martin Pacesa, Chun-Han Lin, Antoine Cléry, Aakash Saha, Pablo R. Arantes, Katja Bargsten, Matthew J. Irby, Frédéric H.-T. Allain, Giulia Palermo, Peter Cameron,
et al.
Dynabeads M-280 Streptavidin
,
Invitrogen
,Cat#
11206D
Q5^ Hot Start High-Fidelty 2x Master Mix,
New England Biolabs
,Cat#
M0494L
NEBNext double-stranded DNA (dsDNA) Fragmentase,
New England Biolabs
,Cat#
M0348L
T4 DNA Ligase
,
New England Biolabs
(76)
Multiple Introductions of SARS-CoV-2 Alpha and Delta Variants into White-Tailed Deer in Pennsylvania
mBio
October 26, 2022
Andrew D. Marques, Scott Sherrill-Mix, John K. Everett, Hriju Adhikari, Shantan Reddy, Julie C. Ellis, Haley Zeliff, Sabrina S. Greening, Carolyn C. Cannuscio, Katherine M. Strelau,
et al.
mixture contains 5 μL of amplicon product from reaction 1, 12.5 μL 2x Q5 hot start master mix (
NEB
,
M0494S
), 0.5 μL of 10 mM dNTPs (
NEB
,
N0447S
), 1.25 μL of each
PCR
2 primers (10 μM), and 7.5 μL water. The
(77)
Engineered helicase replaces thermocycler in DNA amplification while retaining desired PCR characteristics
Nature Communications
October 23, 2022
MomÄilo Gavrilov, Joshua Y. C. Yang, Roger S. Zou, Wen Ma, Chun-Ying Lee, Sonisilpa Mohapatra, Jimin Kang, Ting-Wei Liao, Sua Myong, Taekjip Ha
samples were amplified with
PCR
using
Q5 Hot Start High-Fidelity 2X Master Mix
(
New England BioLabs
M0494
) in 10 µL reactions (3 µL water, 1 µL 10 uM primer mixture, 1 µL genomic DNA, 5 µL 2x master mix). Primer
(78)
Innate type 2 immunity controls hair follicle commensalism by Demodex mites
Immunity
October 11, 2022
Ricardo-Gonzalez RR, Kotas ME, O'Leary CE, Singh K, Damsky W, Liao C, Arouge E, Tenvooren I, Marquez DM, Schroeder AW,
et al
.
Technologies,Cat#14190-250 191/193Ir DNA inercalator,Fluidigm,Cat#201192B
Q5 High-Fidelity DNA Polymerase
,
NEB
,
M0494L
Yeast
cell lysis
buffer,Lucigen,Cat#MPY80200
Lucifer yellow
,
ThermoFisher
,Cat#
L453
Optimal Cutting
(79)
Optimized In Vitro CRISPR/Cas9 Gene Editing Tool in the West Nile Virus Mosquito Vector, Culex quinquefasciatus
Insects
September 19, 2022
Tran Zen B. Torres, Brian C. Prince, Alexis Robison, Claudia Rückert
Drosophila
-optimized plasmid. PCRs were carried out with Q5® Hot Start High-Fidelity Master Mix (
New England Biolabs
, #
M0494
). Multiple overlapping amplicons were assembled using
NEBuilder HiFi DNA Assembly Master Mix
(
New England Biolabs
(80)
Protocol for construction and characterization of direct electron transfer-based enzyme-electrode using gold binding peptide as molecular binder
STAR Protocols
September 16, 2022
Hyeryeong Lee, Eun Mi Lee, Stacy Simai Reginald, In Seop Chang
recombinant proteins" ,, Q5® Hot Start High-Fidelity 2× Master Mix,"New England Biolabs (Ipswich, MA, USA)",
M0494S
KLD enzyme mix
,"
New England Biolabs
(Ipswich, MA, USA)",
M0554S
Expin™
PCR
SV,"GeneAll (Seoul, South
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Reviews
Write a Review
(1)
10/6/2023
Convenient PCR Master Mix From New England Biolabs
Anh Nguyen
I used this Master Mix for running PCR which contained all the enzymes and reagents needed. I only needed to add primers, a DNA template, and water to this Master Mix for PCR reactions.
(1)
3/31/2023
Good PCR Master Mix
YONG M
Try this master mix for hard PCR application from genomic DNA. It's simple to use and the mix includes every component. The enzyme has high fidelity and could amplify the difficult fragment.
(1)
1/2/2020
DNA Insert Amplification for Cloning
Dr. Thippeswamy Sannasiddappa
Highly recommended for amplifying error free longer length DNA inserts. Also, reactions can be set up room temperature.
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Q5 Hot Start High-Fidelity 2X Master Mix from New England Biolabs
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
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