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PCR Master Mixes (High Fidelity)
Q5 Hot Start High-Fidelity 2X Master Mix
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Q5 Hot Start High-Fidelity 2X Master Mix
Q5 Hot Start High-Fidelity 2X Master Mix from New England Biolabs
(3)
Write a review
Citations:
(255)
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Supplier Page from
New England Biolabs for
Q5 Hot Start High-Fidelity 2X Master Mix
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Q5 Hot Start High-Fidelity 2X Master Mix
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0494S
Quantity
100 reactions
Applications
DNA Analysis
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Master Mix
Temperature
-20 C
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (255)
(41)
mRNA targeting eliminates the need for the signal recognition particle during membrane protein insertion in bacteria
Cell Reports
March 28, 2023
Pinku Sarmah, Wenkang Shang, Andrea Origi, Mariya Licheva, Claudine Kraft, Maximilian Ulbrich, Elisabeth Lichtenberg, Annegret Wilde, Hans-Georg Koch
Kit,
Lucigen
,Cat#AS3107
T4-Polynucleotide-Kinase
,
Thermo-Fisher
,Cat#
EK0031
Gibson HiFi Assembly Master Mix,
NEB
,Cat#
E2611L
Q5 hot start high-fidelity 2X master mix
,
NEB
,Cat#
M0494S
KLD enzyme mix
,
NEB
,Cat#
M0554S
Q5
Site directed mutagenesis
,
NEB
,Cat#
E0554S
,, Oligonucleotides,Oligonucleotides,Oligonucleotides ,, SeeTable
(42)
The potential to produce tropodithietic acid by Phaeobacter inhibens affects the assembly of microbial biofilm communities in natural seawater
npj Biofilms and Microbiomes
March 23, 2023
Pernille Kjersgaard Bech, Sheng-Da Zhang, Nathalie Nina Suhr Eiris Henriksen, Mikkel Bentzon-Tilia, Mikael Lenz Strube, Lone Gram
primers PtdaB-Pinh and GFPmut3-Rv. The Q5 hot start high fidelity 2 × master mix (
New England Biolabs
,
M0494S
, BioNordika, Denmark) was used for the reactions. The fused PtdaCDE-gfp segments were cloned between
(43)
Rabies virus-based barcoded neuroanatomy resolved by single-cell RNA and in situ sequencing
bioRxiv
March 22, 2023
Aixin Zhang, Lei Jin, Shenqin Yao, Makoto Matsuyama, Cindy van Velthoven, Heather Sullivan, Na Sun, Manolis Kellis, Bosiljka Tasic, Bosiljka Tasic,
et al.
AGCAATCTACGGATTGTGTATATCCATC The
long-range PCR
was performed as described below:
Q5® Hot Start High-Fidelity 2X Master Mix
(
M0494S
,
NEB
): 12.5 μL; N_Barcode_160bp_rp_57 (10uM): 1.25 μL; N_Barcode_NheI_fp_58 (10uM): 1.25 μL; pRVΔG-4mCherry
(44)
Autocatalytic base editing for RNA-responsive translational control
Nature Communications
March 11, 2023
Raphaël V. Gayet, Katherine Ilia, Shiva Razavi, Nathaniel D. Tippens, Makoto A. Lalwani, Kehan Zhang, Jack X. Chen, Jonathan C. Chen, Jose Vargas-Asencio, James J. Collins
DNA assembly mix (
NEB
#
E2621
). We generated fragments by
PCR
using high-fidelity Q5 polymerase (
NEB
#
M0494
), with in-house plasmids and custom-synthesized gBlocks (Integrated DNA Technologies) as templates.
(45)
Differential Occupancy and Regulatory Interactions of KDM6A in Bladder Cell Lines
Cells
March 8, 2023
Gülden Özden-Yılmaz, Busra Savas, Ahmet Bursalı, Aleyna Eray, Alirıza Arıbaş, Serif Senturk, Ezgi Karaca, Gökhan Karakülah, Serap Erkek-Ozhan
from this vector. In order to add a FLAG-tag to
KDM6A
, 2-step
PCR
was set up using the Q5 polymerase (
M0494S
,
Neb
, Ipswich, MA, USA) with designed primers (see primer list, Table S3). The
PCR product
was cut from
(46)
Generation of two human iPSC lines with Exon 3 mutations in BCL2-Associated Athanogene 3 (BAG3) from dilated cardiomyopathy patients
Stem cell research
March 1, 2023
Zushin PH, Zhou Y, Li A, Ashley EA, Wheeler MT, Wu JC.
designed in Benchling to flank exon 3 (Table 2) and used with
Q5® Hot Start High-Fidelity DNA polymerase
(
M0494L
,
NEB
). The
PCR
was performed according to manufacturer recommendation with a 69°C annealing temp.
PCR
(47)
A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis Bullosa
bioRxiv : the preprint server for biology
March 1, 2023
Neumayer G, Torkelson JL, Li S, McCarthy K, Zhen HH, Vangipuram M, Jackow J, Rami A, Hansen C, Guo Z,
et al
.
using the primers outlined in Table S1 and Q5 Hot-start high fidelity polymerase (
New England Biolabs
M0494S
).
PCR
products were extracted from standard agarose gels using the QIAquick Gel Extraction Kit (
Qiagen
(48)
The MYC-regulated lncRNA LNROP (ENSG00000254887) enables MYC-driven cell proliferation by controlling the expression of OCT2
Cell Death & Disease
February 27, 2023
Daniel García-Caballero, Jonathan R. Hart, Peter K. Vogt
binding sites and barcodes (Table S2). The
Q5 Hot Start High-Fidelity 2X Master Mix
(
New England Biolabs
;
M0494
) was used for
amplification
, with a total of 23
PCR
cycles at an annealing temperature of 61 °C. The
(49)
Rapid whole genome sequencing methods for RNA viruses
Frontiers in Microbiology
February 23, 2023
Masayasu Misu, Tomoki Yoshikawa, Satoko Sugimoto, Yuki Takamatsu, Takeshi Kurosu, Yukiteru Ouji, Masahide Yoshikawa, Masayuki Shimojima, Hideki Ebihara, Masayuki Saijo
extension of 68°C for 2 min. Alternatively,
Q5 Hot Start High-Fidelity 2X Master Mix
(catalog number
M0494S
,
New England BioLabs
) was used to determine the whole genome sequences of CDV and
SARS-CoV-2
instead
(50)
The Pathfinder plasmid toolkit for genetically engineering newly isolated bacteria enables the study of Drosophila -colonizing Orbaceae
bioRxiv
February 19, 2023
Katherine M. Elston, Laila E. Phillips, Sean P. Leonard, Eleanor Young, Jo-anne C. Holley, Tasneem Ahsanullah, Braydin McReynolds, Nancy A. Moran, Jeffrey E. Barrick
Integrated DNA Technologies and then amplified
PCR
products using either Q5 Hot-Start Master Mix (#
M0494S
,
New England Biolabs
) or KOD XL (#
71087–3
,
Millipore Sigma
) according to manufacturer’s instructions.
(51)
Olivar: fully automated and variant aware primer design for multiplex tiled amplicon sequencing of pathogen genomes
bioRxiv
February 17, 2023
Michael X. Wang, Esther G. Lou, Nicolae Sapoval, Eddie Kim, Prashant Kalvapalle, Bryce Kille, R. A. Leo Elworth, Yunxi Liu, Yilei Fu, Lauren B. Stadler,
et al.
of Olivar).
PCR amplification
was also performed using
Q5 Hot Start High-Fidelity 2X Master Mix
(
NEB
M0494
) as described in ref. 17.
PCR
products were purified using AmPureTM XP beads (
Beckman Coulter Inc
.,
(52)
Transcriptionally active nasopharyngeal commensals and opportunistic microbial dynamics define mild symptoms in the COVID 19 vaccination breakthroughs
PLOS Pathogens
February 17, 2023
Priti Devi, Pallawi Kumari, Aanchal Yadav, Bansidhar Tarai, Sandeep Budhiraja, Uzma Shamim, Rajesh Pandey
primers (IDT Product number: 10007184) and
Q5 High-Fidelity 2X master mix
(
New England Biolabs
, Cat. No.
M0494S
). For
sequencing
, the amplified products were ligated with rapid barcode sequences (SQK-RBK110.96) followed
(53)
Functional investigation of inherited noncoding genetic variation impacting the pharmacogenomics of childhood acute lymphoblastic leukemia treatment
medRxiv
February 14, 2023
Kashi Raj Bhattarai, Robert J. Mobley, Kelly R. Barnett, Daniel C. Ferguson, Baranda S. Hansen, Jonathan D. Diedrich, Brennan P. Bergeron, Wenjian Yang, Kristine R. Crews, Christopher S. Manring,
et al.
independently prepared for
next-generation sequencing
) was carried out using Q5 Hot Start 2x Master Mix (
NEB
#
M0494S
), index primers, and
Multiplexing
primer 1 for 15 cycles of
PCR
. The reactions were size selected using
(54)
Systematic identification and characterization of repressive domains in Drosophila transcription factors
The EMBO Journal
February 1, 2023
Loni Klaus, Bernardo P de Almeida, Anna Vlasova, Filip Nemčko, Alexander Schleiffer, Katharina Bergauer, Lorena Hofbauer, Martina Rath, Alexander Stark
μm ID, 2 μm, 100 Å",Thermo Fisher Scientific,164942
Q5® Hot Start High‐Fidelity 2X Master Mix
,
NEB
,
M0494L
T7 RNA Polymerase
,
Promega
,
P2075
Oligo(dt)20 primer
,
Invitrogen
,
18418020
Promega
GoTaq qPCR Master Mix
(55)
SPRTN patient variants cause global-genome DNA-protein crosslink repair defects
Nature communications
January 21, 2023
Weickert P, Li HY, Götz MJ, Dürauer S, Yaneva D, Zhao S, Cordes J, Acampora AC, Forne I, Imhof A,
et al
.
TEDA-based cloning47 into a pDONR221 backbone amplified with
Q5® Hot Start High-Fidelity 2X Master Mix
(
M0494S
,
NEB
). Plasmid DNA was isolated from at least five single colonies and analysed by
Sanger sequencing
. siRNA
(56)
Genetic conversion of a split-drive into a full-drive element
Nature Communications
January 12, 2023
Gerard Terradas, Jared B. Bennett, Zhiqian Li, John M. Marshall, Ethan Bier
Plasmid and genomic DNA sequences were amplified using Q5 Hotstart Master Mix (
New England Biolabs
, Cat. #
M0494S
) and Gibson assembled with
NEBuilder HiFi DNA Assembly Master Mix
(
New England Biolabs
, Cat. #
E2621
).
(57)
Improved targeting of the 16S rDNA nanopore sequencing method enables rapid pathogen identification in bacterial pneumonia in children
Frontiers in Cellular and Infection Microbiology
January 9, 2023
Yinghu Chen, Lingfeng Mao, Dengming Lai, Weize Xu, Yuebai Zhang, Sihao Wu, Di Yang, Shaobo Zhao, Zhicong Liu, Yi Xiao,
et al.
5’-barcode-1492R-CGGTTACC TTGTTACGACTT-3’) with 25 μL of
Q5 Hot Start High-Fidelity 2X Master Mix
(
M0494L
,
New England Biolabs
, USA) and 14.5 μL of nuclease-free water (New England Biolabs, USA).
PCR
amplicons
(58)
Identification of Genes Associated with Liver Metastasis in Pancreatic Cancer Reveals PCSK6 as a Crucial Mediator
Cancers (Basel)
December 30, 2022
Hang He, Shuman Zhang, Hai Yang, Pengyan Xu, Isabella Kutschick, Susanne Pfeffer, Nathalie Britzen-Laurent, Robert Grützmann, Deliang Fu, Christian Pilarsky
obtained genomic DNA were performed with
Q5® Hot Start High-Fidelity 2X Master Mix
(
New England Biolabs
,
M0494L
, Frankfurt, Germany) according to the manufacturer’s instructions and primers for sgRNAs targeting regions
(59)
CRISPR‐Cas9 Genome Editing and Rapid Selection of Cell Pools
Current Protocols
December 22, 2022
Daniel Stoyko, Timothy Timothy, Adrianna Hernandez, Parthena Konstantinidou, Qingcai Meng, Astrid D. Haase
Zymo Research Catalog #
D4001
Q5 Hot Start High-Fidelity 2x Master Mix
-
New England BioLabs
Catalog #
M0494
(or alternative
PCR kit
) LB Liquid Broth (Engebrecht et al., 2001) Ampicillin – Sigma Aldrich Catalog
(60)
Protocols for genetic labeling and tracing of Staphylococcus xylosus during tumor progression
STAR Protocols
December 16, 2022
Tingting Dong, Aikun Fu, Shang Cai
Zymoclean Gel DNA Recovery Kit
(200 Preps),
Zymo Research
,
D4008
Q5® Hot Start High-Fidelity 2× Master Mix,
NEB
,
M0494S
2× Es Taq master mix (Dye),CW bio,CW0690L
Gibson Assembly® Master Mix
,
NEB
,
E2611S
Plasmid extraction
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Reviews
Write a Review
(1)
10/6/2023
Convenient PCR Master Mix From New England Biolabs
Anh Nguyen
I used this Master Mix for running PCR which contained all the enzymes and reagents needed. I only needed to add primers, a DNA template, and water to this Master Mix for PCR reactions.
(1)
3/31/2023
Good PCR Master Mix
YONG M
Try this master mix for hard PCR application from genomic DNA. It's simple to use and the mix includes every component. The enzyme has high fidelity and could amplify the difficult fragment.
(1)
1/2/2020
DNA Insert Amplification for Cloning
Dr. Thippeswamy Sannasiddappa
Highly recommended for amplifying error free longer length DNA inserts. Also, reactions can be set up room temperature.
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Q5 Hot Start High-Fidelity 2X Master Mix from New England Biolabs
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
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