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PCR Master Mixes (High Fidelity)
Q5 Hot Start High-Fidelity 2X Master Mix
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Q5 Hot Start High-Fidelity 2X Master Mix
Q5 Hot Start High-Fidelity 2X Master Mix from New England Biolabs
(3)
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Citations:
(253)
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New England Biolabs for
Q5 Hot Start High-Fidelity 2X Master Mix
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Company Info
Citations
Reviews
Product Specs
Item
Q5 Hot Start High-Fidelity 2X Master Mix
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0494S
Quantity
100 reactions
Applications
DNA Analysis
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Master Mix
Temperature
-20 C
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (253)
(21)
Targeting Sex Determination to Suppress Mosquito Populations
Research Square
July 27, 2023
Omar Akbari, Ming Li, Nikolay Kandul, Ruichen Sun, Ting Yang, Elena Dalla Dalla Benetta, Daniel Brogan, igor antoshechkin, Héctor Sánchez Sánchez C, Yin Peng Zhan,
et al.
(Integrated DNA Technologies) using Q5 Hotstart Start High-Fidelity 2X Master Mix (
New England Biolabs
, Cat. #
M0494S
). The generated plasmids were transformed into Zymo JM109 chemically competent
E. coli
(
Zymo Research
,
(22)
Regulation of store-operated Ca2+ entry by IP3 receptors independent of their ability to release Ca2+
eLife
July 19, 2023
Pragnya Chakraborty, Bipan Kumar Deb, Vikas Arige, Thasneem Musthafa, Sundeep Malik, David I Yule, Colin W Taylor, Gaiti Hasan
modifications were carried out using Q5 Hot Start high-fidelity 2 X Master Mix (
New England BioLabs
, Cat#
M0494L
) using the recommendations of the manufacturer. Primers used in this study (details given in Supplementary
(23)
SPIDR: a highly multiplexed method for mapping RNA-protein interactions uncovers a potential mechanism for selective translational suppression upon cellular stress
bioRxiv
July 17, 2023
Erica Wolin, Jimmy K. Guo, Mario R. Blanco, Andrew A. Perez, Isabel N. Goronzy, Ahmed A. Abdou, Darvesh Gorhe, Mitchell Guttman, Marko Jovanovic
separate tube. Biotinylated oligo tags were amplified on-bead using 2X Q5 Hot-Start Mastermix (
NEB
#
M0494
) with primers that add the indexed full Illumina adaptor sequences. To isolate barcoded cDNA, the supernatant
(24)
Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathy
Nature Communications
June 22, 2023
Markus Grosch, Laura Schraft, Adrian Chan, Leonie Küchenhoff, Kleopatra Rapti, Anne-Maud Ferreira, Julia Kornienko, Shengdi Li, Michael H. Radke, Chiara Krämer,
et al.
Nextera-compatible adapters (Supplementary Data 6) using Q5® Hot Start High-Fidelity 2× Master Mix (
M0494L
,
NEB
). One microliter of a
1:100
dilution was used for a second
PCR
attaching sample-specific index
(25)
Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat production
bioRxiv
June 19, 2023
Andrew J. Stout, Xiaoli Zhang, Sophia M. Letcher, Miriam L. Rittenberg, Michelle Shub, Kristin M. Chai, Maya Kaul, David L. Kaplan
performed via gibson assemblies. This involved
amplification
with
Q5 high-fidelity DNA polymerase
(
NEB
#
M0494S
, Ipswich, MA, USA),
DpnI
digestion (
NEB
#
R0176S
),
PCR
cleanup (
NEB
#
T1030S
), assembly with the NEBuilder
(26)
Calling Cards: a customizable platform to longitudinally record protein-DNA interactions over time in cells and tissues
bioRxiv
June 15, 2023
Allen Yen, Chase Mateusiak, Simona Sarafinovska, Mariam A. Gachechiladze, Juanru Guo, Xuhua Chen, Arnav Moudgil, Alexander J. Cammack, Jessica Hoisington-Lopez, MariaLynn Crosby,
et al.
distribution of barcodes that were packaged. Materials: Q5 Hot Start High-Fidelity Master Mix (
NEB
M0494
) AMPure XP Reagent (Beckman Coulter
A63882
) or Mag-Bind TotalPure
NGS
beads (Omega Biotek M1378-02) NOTE:
(27)
A self‐propagating, barcoded transposon system for the dynamic rewiring of genomic networks
Molecular Systems Biology
June 12, 2023
Max A English, Miguel A Alcantar, James J Collins
Standard Buffer,
New England Biolabs
,
M0484L
Q5® Hot Start High‐Fidelity 2X Master Mix
,
New England Biolabs
,
M0494L
BbsI‐HF
,
New England Biolabs
,
R3539L
BsmBI‐v2
,
New England Biolabs
,
R0739L
FseI
,
New England Biolabs
,
R0588L
SbfI‐HF
,
New England Biolabs
(28)
The Pathfinder plasmid toolkit for genetically engineering newly isolated bacteria enables the study of Drosophila-colonizing Orbaceae
ISME Communications
May 24, 2023
Katherine M. Elston, Laila E. Phillips, Sean P. Leonard, Eleanor Young, Jo-anne C. Holley, Tasneem Ahsanullah, Braydin McReynolds, Nancy A. Moran, Jeffrey E. Barrick
Integrated DNA Technologies and then amplified
PCR
products using either Q5 Hot-Start Master Mix (#
M0494S
,
New England Biolabs
) or KOD XL (#
71087-3
,
Millipore Sigma
) according to manufacturer’s instructions.
(29)
The ATP Synthase γ Subunit ATPC1 Regulates RNA Editing in Chloroplasts
International Journal of Molecular Sciences
May 24, 2023
Jia Ni, Wenjian Song, Nadia Ahmed Ali, Yayi Zhang, Jiani Xing, Kexing Su, Xingxing Sun, Xiaobo Zhao
coding sequence of the 3×FLAG tag was amplified by
PCR
using Q5 Hot Start High-Fidelity 2×Master Mix (
M0494S
,
NEB
, Ipswich, MA, USA). The following thermal condition was used: 98 °C for 3 min, 33 cycles of 98
(30)
A new method to synthesize multiple gRNA libraries and functional mapping of mammalian H3K4me3 regions
Nucleic Acids Research
May 22, 2023
Chen Pan, Ran Li, Liyan Shui, Zhengyun Xiao, Yali Wang, Jing Zhu, Chao Wu, Long Zhang, Junling Jia, Min Zheng
designed via NCBI Primer BLAST, and then
PCR
was conducted with Q5 Hot Start High-Fidelity 2× Mix (
NEB
,
M0494
) (Supplementary Table S11). The
PCR product
was purified via a QIAquick PCR Clean-up Kit and was constructed
(31)
HPV integration generates a cellular super-enhancer which functions as ecDNA to regulate genome-wide transcription
Nucleic Acids Research
May 22, 2023
Rui Tian, Zheying Huang, Lifang Li, Jingping Yuan, Qinghua Zhang, Lirong Meng, Bin Lang, Yongfeng Hong, Chaoyue Zhong, Xun Tian,
et al.
polymerase chain reaction (PCR
) was conducted using the
Q5 Hot Start High-Fidelity 2X Master Mix
(
NEB
,
M0494S
). The primers are listed in Supplementary Table S3.
RT–qPCR
Total RNA was extracted by RNA-easy Isolation
(32)
High-resolution spatial multi-omics reveals cell-type specific nuclear compartments
bioRxiv
May 12, 2023
Yodai Takei, Yujing Yang, Jonathan White, Jina Yun, Meera Prasad, Lincoln J Ombelets, Simone Schindler, Long Cai
from 13 different oligo array pools. Briefly, we performed
PCR
with Q5 Hot Start High-Fidelity (
NEB
M0494S
), column
purification
with QIAquick PCR Purification Kit (
Qiagen
28104
),
in vitro transcription
(
NEB
(33)
DNA rehybridization drives product release from Cas9 ribonucleoprotein to enable multiple-turnover cleavage
Nucleic Acids Research
May 8, 2023
Juan Pan, Megumu Mabuchi, Gregory Brett Robb
Monarch® Total RNA Miniprep Kit
(#
T2010S
), Monarch®
PCR
& DNA Cleanup Kit (#
T1030L
), Q5® Hot Start High-Fidelity 2× Master Mix (#
M0494L
),
EcoRI-HF
(#
R3101S
), rSAP (#
M0371S
),
T4 DNA ligase
(#
M0202S
),
NEB
® 10-beta Competent
Escherichia coli
(34)
Improved cytosine base editors generated from TadA variants
Nature Biotechnology
May 1, 2023
Dieter K. Lam, Patricia R. Feliciano, Amena Arif, Tanggis Bohnuud, Thomas P. Fernandez, Jason M. Gehrke, Phil Grayson, Kin D. Lee, Manuel A. Ortega, Courtney Sawyer,
et al.
Multiplex PCR Master Mix (
Thermo Fisher Scientific
,
F564L
),
Q5 Hot Start High-Fidelity 2X Master Mix
(
NEB
,
M0494L
), Mach T1 competent cells (
Thermo Fisher Scientific
,
C8681201
) and ZymoPURE II Plasmid
Midiprep
kits
(35)
Improving the sensitivity of in vivo CRISPR off-target detection with DISCOVER-Seq
Nature methods
May 1, 2023
Zou RS, Liu Y, Gaido OER, Konig MF, Mog BJ, Shen LL, Aviles-Vazquez F, Marin-Gonzalez A, Ha T.
samples were amplified with
PCR
using Q5 HotStart High-Fidelity 2X Master Mix (
New England BioLabs
,
M0494
). Primer pairs for all sequences are listed in Supplementary Table 3. For example, the primer set for
(36)
CRISPR/Cas9-Mediated Knockout of tnfaip1 in Zebrafish Plays a Role in Early Development
Genes (Basel)
April 28, 2023
Shulan Huang, Hongning Zhang, Wen Chen, Na Su, Changyue Yuan, Jian Zhang, Shuanglin Xiang, Xiang Hu
the antisense RNA probe template for
PCR amplification
(Q5® Hot Start High-Fidelity 2× Master Mix,
M0494
,
NEB
, Ipswich, MA, USA) with a cDNA template from 72 hpf wild-type
zebrafish
embryos. Digoxigenin-labelled
(37)
Late Embryogenesis Abundant Proteins Contribute to the Resistance of Toxoplasma gondii Oocysts against Environmental Stresses
mBio
April 25, 2023
David Arranz-Solís, David Warschkau, Benedikt T. Fabian, Frank Seeber, Jeroen P. J. Saeij
conducted by using either
Q5 Hot Start High-Fidelity DNA polymerase
(
New England Biolabs
, cat. no.
M0494S
) for
cloning
purposes, or MangoMix (Meridian Bioscience ref. BIO-25034) for diagnostic/
screening
purposes.
(38)
Targeting Sex Determination to Suppress Mosquito Populations
bioRxiv
April 23, 2023
Ming Li, Nikolay P. Kandul, Ruichen Sun, Ting Yang, Elena D. Benetta, Daniel J. Brogan, Igor Antoshechkin, Héctor M. Sánchez C., Yinpeng Zhan, Nicolas A. DeBeaubien,
et al.
(Integrated DNA Technologies) using Q5 Hotstart Start High-Fidelity 2X Master Mix (
New England Biolabs
, Cat. #
M0494S
). The generated plasmids were transformed into Zymo JM109 chemically competent
E. coli
(
Zymo Research
,
(39)
Dynamics of Age- versus Therapy-Related Clonal Hematopoiesis in Long-term Survivors of Pediatric Cancer
Cancer Discovery
April 3, 2023
Kohei Hagiwara, Sivaraman Natarajan, Zhaoming Wang, Haseeb Zubair, Heather L. Mulder, Li Dong, Emily M. Plyler, Padma Thimmaiah, Xiaotu Ma, Kristen K. Ness,
et al.
region was amplified by
PCR
using Q5 Hot Start High-Fidelity 2 × Master Mix (
New England BioLabs
, #
M0494L
). Two primer sets were used to generate the amplicons. The first set included the forward primer GGAAAGAAAAAATGGGCAG
(40)
mRNA targeting eliminates the need for the signal recognition particle during membrane protein insertion in bacteria
Cell Reports
March 28, 2023
Pinku Sarmah, Wenkang Shang, Andrea Origi, Mariya Licheva, Claudine Kraft, Maximilian Ulbrich, Elisabeth Lichtenberg, Annegret Wilde, Hans-Georg Koch
Kit,
Lucigen
,Cat#AS3107
T4-Polynucleotide-Kinase
,
Thermo-Fisher
,Cat#
EK0031
Gibson HiFi Assembly Master Mix,
NEB
,Cat#
E2611L
Q5 hot start high-fidelity 2X master mix
,
NEB
,Cat#
M0494S
KLD enzyme mix
,
NEB
,Cat#
M0554S
Q5
Site directed mutagenesis
,
NEB
,Cat#
E0554S
,, Oligonucleotides,Oligonucleotides,Oligonucleotides ,, SeeTable
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Reviews
Write a Review
(1)
10/6/2023
Convenient PCR Master Mix From New England Biolabs
Anh Nguyen
I used this Master Mix for running PCR which contained all the enzymes and reagents needed. I only needed to add primers, a DNA template, and water to this Master Mix for PCR reactions.
(1)
3/31/2023
Good PCR Master Mix
YONG M
Try this master mix for hard PCR application from genomic DNA. It's simple to use and the mix includes every component. The enzyme has high fidelity and could amplify the difficult fragment.
(1)
1/2/2020
DNA Insert Amplification for Cloning
Dr. Thippeswamy Sannasiddappa
Highly recommended for amplifying error free longer length DNA inserts. Also, reactions can be set up room temperature.
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Q5 Hot Start High-Fidelity 2X Master Mix from New England Biolabs
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