Welcome Guest
Sign In
Register
Products
Popular Categories
Antibody Search
ELISA Kit Search
Biomolecules
Flow Cytometers
Thermal Cyclers
Microscopes
Transfection Reagents
Featured Products
New Products
Articles
Resources
eBooks
Bench Tips
Tech Insights
Editorial Articles
News
Product Reviews
Future Lab
Multimedia
Biocompare Webinars
Immunotherapy
Reproducibility
Transformative Technologies
Podcasts
Tech Talks
Bench Tip Videos
All Videos and Webinars
Life Science Webinars
Write a Review
Antibodies
Kits/Reagents
Equipment
Services
Resources
Promotions
Events
AACR SPOTLIGHT
Register
Sign In
Biocompare
Products
Cell Biology
Biomolecules
Biomolecules
T4 Polynucleotide Kinase
Supplier Images
T4 Polynucleotide Kinase
T4 Polynucleotide Kinase from New England Biolabs
(1)
Write a review
Citations:
(>1k)
Supplier Page
Supplier Page from
New England Biolabs for
T4 Polynucleotide Kinase
Get Pricing
Description
T4 Polynucleotide Kinase
Product Specs
Company Info
Citations
Reviews
Protocols
Product Specs
Item
T4 Polynucleotide Kinase
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0201S
Format
10 u/µl
Molecule Name
Polynucleotide Kinase
Activity
One Richardson unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmole of acid-insoluble [32P] in 30 minutes at 37°C.
Quantity
500 units
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Enzymes
Temperature
-20 C
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (1124)
(41)
Nanopore-based RNA sequencing deciphers the formation, processing, and modification steps of rRNA intermediates in archaea
RNA
August 1, 2023
Felix Grünberger, Michael Jüttner, Robert Knüppel, Sébastien Ferreira-Cerca, Dina Grohmann
sequencing
RNAs for direct cDNA and
PCR
-cDNA
sequencing
were treated with
T4 Polynucleotide Kinase
(
NEB
,
M0201
) following the manufacturer's nonradioactive phosphorylation protocol to dephosphorylate 3′ termini,
(42)
RNA polymerase II dynamics shape enhancer–promoter interactions
Nature Genetics
August 1, 2023
Gilad Barshad, James J. Lewis, Alexandra G. Chivu, Abderhman Abuhashem, Nils Krietenstein, Edward J. Rice, Yitian Ma, Zhong Wang, Oliver J. Rando, Anna-Katerina Hadjantonakis,
et al.
for 10 min. Following dephosphorylation with rSAP (
NEB
#
M0371
) and end polishing using T4 PNK (
NEB
#
M0201
), DNA polymerase Klenow fragment (
NEB
#
M0210
) and biotinylated dATP and dCTP (Jena Bioscience #NU-835-BIO14-S
(43)
METTL1 promotes tumorigenesis through tRNA-derived fragment biogenesis in prostate cancer
Molecular Cancer
July 29, 2023
Raquel García-Vílchez, Ana M. Añazco-Guenkova, Sabine Dietmann, Judith López, Virginia Morón-Calvente, Silvia D’Ambrosi, Paz Nombela, Kepa Zamacola, Isabel Mendizabal, Saioa García-Longarte,
et al.
(
ThermoFisher Scientific
, #
88836
) were used. After washing, RNA 3’ ends were dephosphorylated using PNK (
NEB
,
M0201L
) and FastAP
Alkaline phosphatase
(
ThermoFisher Scientific
,
EF0654
). Next, the 3’ pre-adenylated adapters
(44)
Aub, Vasa and Armi localization to phase separated nuage is dispensable for piRNA biogenesis and transposon silencing in Drosophila
bioRxiv
July 29, 2023
Samantha Ho, Nicholas P Rice, Tianxiong Yu, Zhiping Weng, William E Theurkauf
I,
NEB
,Cat#
M0209S
T4 DNA polymerase
,
NEB
,Cat#
M0203L
Klenow DNA polymerase,
NEB
,Cat#
M0210S
T4 PNK,
NEB
,Cat#
M0201L
Klenow 3ʹ to 5ʹ exo,NEB,Cat#
M0212L
UDG,
NEB
,Cat#
M0280S
Phusion Polymerase,
NEB
,Cat#
M0530S
"
T4 RNA Ligase 2, truncated
(45)
Activation of GPR44 decreases severity of myeloid leukemia via specific targeting of leukemia initiating stem cells
Cell reports
July 25, 2023
Qian F, Nettleford SK, Zhou J, Arner BE, Hall MA, Sharma A, Annageldiyev C, Rossi RM, Tukaramrao DB, Sarkar D,
et al
.
T4 DNA ligase
,
New England Biolabs
,Cat#
M0202S
T4 Polynucleotide Kinase
(500 units),
New England Biolabs
,Cat#
M0201S
Torin 1
,
Selleckchem
,Cat#
S2827
TransIT®-293 Reagent,MirusBio,Cat# MIR 2705
TRI Reagent
®,
Sigma Aldrich
,Cat#
(46)
Genome-wide CRISPR-Cas9 screen analyzed by SLIDER identifies network of repressor complexes that regulate TRIM24
iScience
July 21, 2023
Patel LR, Stratton SA, McLaughlin M, Krause P, Allton K, Rivas AL, Barbosa D, Hart T, Barton MC.
Pyruvate,Gibco,
S8636
Geneticin,
Sigma
,
A1720
0.05% trypsin/0.53mM
EDTA
,
Gibco
,25300-054 FBS,Gibco,A31605 DMSO,
Sigma
,
D8418
T4 PNK,
NEB
,
M0201S
CutSmart Buffer
,
NEB
,B7204
Esp3I
,
Thermo
,
ER0452
EcoRI
,
NEB
,
R0101S
BamHI
,
NEB
,
R0136S
T4 Ligase,
NEB
,
M0202S
XbaI
,
NEB
,
R0145S
HindIII
,
NEB
,
R0104S
Q5
(47)
Efficient and sensitive profiling of RNA–protein interactions using TLC-CLIP
Nucleic Acids Research
July 21, 2023
Christina Ernst, Julien Duc, Didier Trono
mM DTT, 10 U SUPERaseIN RNase Inhibitor (
ThermoFisher
, #AM2696), 5 U
T4 Polynucleotide Kinase
(
NEB
, #
M0201L
) for 20 min at 37°C. Beads were washed once in PNK Wash Buffer and resuspended in 20 μl of
ligation
(48)
Mechanisms of readthrough mitigation reveal principles of GCN1-mediated translational quality control
Cell
July 20, 2023
Martin B.D. Müller, Prasad Kasturi, Gopal G. Jayaraj, F. Ulrich Hartl
excised from a 15% TBE-urea gel. The recovered RNA fragments were dephosphorylated with T4 PNK (
NEB
,
M0201S
) and ligated to a DNA barcoded linker using T4 Rnl2(tr) K227Q (
NEB
,
M0351L
). Non-ligated linkers were
(49)
SPIDR: a highly multiplexed method for mapping RNA-protein interactions uncovers a potential mechanism for selective translational suppression upon cellular stress
bioRxiv
July 17, 2023
Erica Wolin, Jimmy K. Guo, Mario R. Blanco, Andrew A. Perez, Isabel N. Goronzy, Ahmed A. Abdou, Darvesh Gorhe, Mitchell Guttman, Marko Jovanovic
RNA were modified to have 3’ OH groups compatible for
ligation
using
T4 Polynucleotide Kinase
(
NEB
, #
M0201L
). Beads were incubated at 37°C for 10 minutes with shaking at 1200 rpm on a ThermoMixer. Following end
(50)
Pleiotropy of autism-associated chromatin regulators
Development (Cambridge, England)
July 15, 2023
Lasser M, Sun N, Xu Y, Wang S, Drake S, Law K, Gonzalez S, Wang B, Drury V, Castillo O,
et al
.
site-directed mutagenesis
using Q5 High Fidelity Master Mix (
NEB
M0492L
),
T4 polynucleotide kinase
(
NEB
M0201L
) and
T4 DNA ligase
(
NEB
M0202L
). Variants were selected by presence in the Satterstrom et al. (2020)
(51)
Single cell susceptibility to SARS-CoV-2 infection is driven by variable cell states
bioRxiv
July 13, 2023
Sam Reffsin, Jesse Miller, Kasirajan Ayyanathan, Margaret C. Dunagin, Naveen Jain, David C. Schultz, Sara Cherry, Arjun Raj
pair by combining the oligos with 1X T4 ligase buffer (
NEB
#
B0202S
) and polynucleotide kinase (
NEB
#
M0201S
) and incubating in a thermocycler (Veriti #4375305) with the following settings: 37°C for 30 min, 95°C
(52)
Relaxed targeting rules help PIWI proteins silence transposons
Nature
July 13, 2023
Ildar Gainetdinov, Joel Vega-Badillo, Katharine Cecchini, Ayca Bagci, Cansu Colpan, Dipayan De, Shannon Bailey, Amena Arif, Pei-Hsuan Wu, Ian J. MacRae,
et al.
Fig. 1a) was obtained from IDT, 5′[32P]-radiolabelled with α-[32P]GTP (Perkin Elmer) and T4 PNK (
NEB
,
M0201
) and purified using a 15% denaturing polyacrylamide gel, extracted with phenol–chloroform–isoamyl alcohol
(53)
Systematic characterization of regulatory variants of blood pressure genes
Cell Genomics
July 12, 2023
Winona Oliveros, Kate Delfosse, Daniella F. Lato, Katerina Kiriakopulos, Milad Mokhtaridoost, Abdelrahman Said, Brandon J. McMurray, Jared W.L. Browning, Kaia Mattioli, Guoliang Meng,
et al.
set,
Qiagen
,Cat#
79254
DNaseI recombinant,Worthington Biochemical,Cat#LS006355
T4 Polynucleotide Kinase
,
NEB
,Cat#
M0201L
SacI-HF
,
NEB
,Cat#
R3156L
Lipofectamine 3000
,
Invitrogen
,Cat#
L3000008
Lipofectamine
Stem
Transfection Reagent
(54)
The Mediator complex regulates enhancer-promoter interactions
Nature Structural & Molecular Biology
July 10, 2023
Shyam Ramasamy, Abrar Aljahani, Magdalena A. Karpinska, T. B. Ngoc Cao, Taras Velychko, J. Neos Cruz, Michael Lidschreiber, A. Marieke Oudelaar
0.4 mM final concentration and 2.5 mM EGTA. Subsequently, 200 U mL–1
T4 polynucleotide Kinase
(
NEB
,
M0201
), 100 U mL–1
DNA polymerase I large (Klenow) fragment
(
NEB
,
M0210
) and 300 U mL–1
T4 DNA ligase
(
Thermo Scientific
(55)
Pyruvate Kinase M (PKM) binds ribosomes in a poly-ADP ribosylation dependent manner to induce translational stalling
Nucleic acids research
July 7, 2023
Kejiou NS, Ilan L, Aigner S, Luo E, Tonn T, Ozadam H, Lee M, Cole GB, Rabano I, Rajakulendran N,
et al
.
phosphorylating primers (antisense to our
hybridization
sites) with [γ32P]
ATP
using
T4 polynucleotide kinase
(
NEB
M0201S
). Probes were then cleaned using Cytiva G-25
MicroSpin Columns
(
Cytiva
27532501
). Extracted RNA was
(56)
Sequential disruption of SPLASH-identified vRNA–vRNA interactions challenges their role in influenza A virus genome packaging
Nucleic Acids Research
July 7, 2023
Celia Jakob, Gabriel L Lovate, Daniel Desirò, Lara Gießler, Redmond P Smyth, Roland Marquet, Kevin Lamkiewicz, Manja Marz, Martin Schwemmle, Hardin Bolte
(122.25 μl H2O, 1.5 μl 10% Tween-20, 15 μl 10x T4 PNK buffer, 3.75 μl Superase-In, 7.5 μl T4 PNK (
NEB
;
M0201L
)). After 4 h of shaking at 37°C, 37.5 μl of phosphorylation mix (3.75 μl 10x T4 PNK buffer, 9.4 μl T4
(57)
Gain of chromosome 21 increases the propensity for P2RY8: :CRLF2 acute lymphoblastic leukemia via increased HMGN1 expression
Frontiers in Oncology
July 6, 2023
Elyse C. Page, Susan L. Heatley, Jacqueline Rehn, Paul Q. Thomas, David T. Yeung, Deborah L. White
37°C. The complementary gRNAs were phosphorylated at a final concentration of 10 µM using T4 PNK (
NEB
#
M0201L
) and then diluted
1:125
with nuclease-free water. Moreover, 5 ng/µL of FgH1tUTG vector was digested
(58)
Biochemical and structural characterization of the first-discovered metazoan DNA cytosine-N4 methyltransferase from the Bdelloid rotifer Adineta vaga
Journal of Biological Chemistry
July 5, 2023
Jujun Zhou, John R. Horton, Gundeep Kaur, Qin Chen, Xuwen Li, Fabian Mendoza, Tao Wu, Robert M. Blumenthal, Xing Zhang, Xiaodong Cheng
oligonucleotides, we extended the length of DNA oligos using
T4 Polynucleotide Kinase
(
New England Biolabs
;
M0201
) and
T4 DNA ligase
(
New England Biolabs
;
M0202
). NAME-seq workflow and data analysis NAME-seq was
(59)
Cooperative control of a DNA origami force sensor
bioRxiv
July 2, 2023
Ariel Robbins, Hazen Hildebolt, Michael Neuhoff, Peter Beshay, Jessica O. Winter, Carlos E. Castro, Ralf Bundschuh, Michael G. Poirier
oligonucleotide to be ligated was phosphorylated using
T4 Polynucleotide Kinase
(T4PNK) (
New England Biolabs
:
M0201
) at 1 U/25 pmol ends incubated at 37 °C for 90 minutes followed by a 65 °C heat
shock
for 20 minutes.
(60)
Histone exchange sensors reveal variant specific dynamics in mouse embryonic stem cells
Nature Communications
June 26, 2023
Marko Dunjić, Felix Jonas, Gilad Yaakov, Roye More, Yoav Mayshar, Yoach Rais, Ayelet-Hashahar Orenbuch, Saifeng Cheng, Naama Barkai, Yonatan Stelzer
mM DTT, 2 mM ATP, 0.8 mM each dATP, dCTP, dGTP, dTTP), 1.25 μl of
T4 polynucleotide kinase
(
NEB
cat#
M0201
) and 0.167 μl of T4 polymerase (
NEB
cat#
M0203
). The samples were incubated 22 min at 12 °C followed
Page
<<
<
1
2
3
4
5
>
>>
Reviews
Write a Review
(1)
4/19/2016
T4 Kinase Kit
Benjamin Gerovac
This kit is used to add 5' phosphate groups to digested DNA so it can be ligated and cloned.
<<
<
>
>>
Protocols
Genome- Scale Mapping of DNase I Hypersensitivity
July 01, 2013
In Vitro Transcription and Immobilized Template Analysis of Preinitiation Complexes
January 01, 2012
In Vitro Selection of RNA Aptamers to a Protein Target by Filter Immobilization
October 01, 2009
Serial Analysis of Gene Expression (SAGE): Experimental Method and Data Analysis
October 01, 2007
Construction of Bacterial Artificial Chromosome (BAC/PAC) Libraries
July 01, 2001
Purification of Sequence- Specific DNA- Binding Proteins by Affinity Chromatography
October 01, 1993
T4 Polynucleotide Kinase from New England Biolabs
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
Protocols
Back To Top