Anti-NDUFAF5 antibodies are used in the immunodetection of the protein NADH:ubiquinone oxidoreductase complex assembly factor 5. In humans, the canonical protein has a reported length of 345 amino acid residues and a mass of 38.9 kDa. Its subcellular localization is in the mitochondria. Up to 2 different isoforms have been reported for this protein. A member of the Methyltransferase protein superfamily, NDUFAF5 is known to be an arginine hydroxylase involved in the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I, MT-ND1) at early stages. The NDUFAF5 gene has been associated with the disease, Mitochondrial complex I deficiency. Synonyms for this target antigen include NADH dehydrogenase (ubiquinone) complex I, assembly factor 5, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5, probable methyltransferase C20orf7, mitochondrial, and arginine-hydroxylase NDUFAF5, mitochondrial. NDUFAF5 gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species.