Anti-Sperm Flagellar 1 antibodies are used for the immunodetection of the protein encoded by the SPEF1 gene. In humans, the canonical protein has a reported length of 236 amino acid residues and a mass of 27 kDa. Its subcellular localization is in the cytoplasm. Alternative splicing is reported to yield 2 different isoforms for this protein. It is reported to be expressed in the intestinal epithelial cells. It is known to be a microtubule-associated protein involved in the stabilization of microtubules along the axis of migration during radial intercalation. Other names for this target antigen include CLAMP, SPEF1A, sperm flagellar protein 1, calponin-homology and microtubule-associated protein, and C20orf28. Gene orthologs have been identified in the mouse, rat, bovine, frog, zebrafish and chimpanzee species. Some Sperm Flagellar 1 antibodies may have been used in research and have associated citations. Western Blot is a widely used application for these antibodies. Immunocytochemistry, Immunofluorescence, and Immunohistochemistry are also common applications.