Anti-Carbonic anhydrase 9 antibodies are used for the immunodetection of the protein encoded by the CA9 gene. In humans, the canonical protein has a reported length of 459 amino acid residues and a mass of 49.7 kDa. Its subcellular localization is in the nucleus and cell membrane. It is notably expressed in carcinoma cells lines. A member of the Alpha-carbonic anhydrase protein family, it is known to catalyze the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. Post-translational modifications have been described, including glycosylation. Other names for this target antigen include MN, CA-IX, P54/58N, RCC-associated antigen G250, RCC-associated protein G250, and CAIX. Gene orthologs have been identified in the mouse, rat, bovine, chimpanzee and chicken species. Over 470 citations in the literature describe the use of Carbonic anhydrase 9 antibodies in research. ELISA, Flow Cytometry, Western Blot, Immunofluorescence, and Immunohistochemistry are common applications for these antibodies.