Anti-ADH4 antibodies are used in the immunodetection of the protein alcohol dehydrogenase 4 (class II), pi polypeptide. In humans, the canonical protein has a reported length of 380 amino acid residues and a mass of 40.2 kDa. Its subcellular localization is in the cytoplasm. Up to 2 different isoforms have been reported for this protein. It is notably expressed in the small intestine, liver, duodenum, and colon. A member of the Zinc-containing alcohol dehydrogenase protein family, ADH4 is known to catalyze the NAD-dependent oxidation of either all-trans-retinol or 9-cis-retinol. Synonyms for this target antigen include HEL-S-4, all-trans-retinol dehydrogenase [NAD(+)] ADH4, alcohol dehydrogenase 2, alcohol dehydrogenase class II pi chain, aldehyde reductase, and ADH-2. ADH4 gene orthologs have been reported in the mouse, bovine and chimpanzee species. A number of ADH4 antibodies have been mentioned in research publications and have associated citations. Western Blot is a widely used application for these antibodies. ELISA and Immunohistochemistry are also common applications.