Anti-Keratin 1 antibodies are used for the immunodetection of the protein encoded by the KRT1 gene. In humans, the canonical protein has a reported length of 644 amino acid residues and a mass of 66 kDa. Its subcellular localization is in the cell membrane and cytoplasm. A member of the Intermediate filament protein family, it is believed to regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). The gene encoding this protein is implicated in Epidermolytic hyperkeratosis. Other names for this target antigen include AEI2 and CK1. Gene orthologs have been identified in the mouse, rat and bovine species.