Fig 1: Proportion of live cells, cellular morphology, and select relative expression of cytobrush-derived endometrial luminal cells. (a) Representative histogram of the proportion of live cells measured by flow cytometry. The y-axis shows the number of cells, and the x-axis indicates the amount of color detected (blue = unstained sample; red = stained sample); (b) optical microscopy of cells harvested immediately after collection by cytobrush (Diff Quick staining); (c, d) optical microscopy of cultured cytobrush-derived cells in early (Fresh) and late (Passage 4) stages of confluence; (e) relative abundance of IFNAR1, KRT18, and VIM during culture progression. Each data point represents one pool (means ± SEM; n = 4 pools, each composed of cells from 2 to 3 animals). *Significantly different from control (Fresh; P < 0.05).