Anti-BUB1 antibodies are used in the immunodetection of the protein BUB1 mitotic checkpoint serine/threonine kinase. In humans, the canonical protein has a reported length of 1085 amino acid residues and a mass of 122.4 kDa. Its subcellular localization is in the nucleus. Up to 3 different isoforms have been reported for this protein. It is reported to be highly expressed in the testis and thymus, less in colon, spleen, lung and small intestine. A member of the Ser/Thr protein kinase protein family, BUB1 is a type of serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Synonyms for this target antigen include BUB1L, MCPH30, hBUB1, mitotic checkpoint serine/threonine-protein kinase BUB1, and BUB1A. BUB1 gene orthologs have been reported in the mouse, rat, bovine, frog, chimpanzee and chicken species. Over 60 citations in the literature describe the use of BUB1 antibodies in research. Western Blot is a widely used application for these antibodies. ELISA, Immunofluorescence, and Immunohistochemistry are also common applications.