Anti-RNA pseudouridine synthase D3 antibodies are used for the immunodetection of the protein encoded by the RPUSD3 gene. In humans, the canonical protein has a reported length of 351 amino acid residues and a mass of 38.5 kDa. Its subcellular localization is in the mitochondria. Alternative splicing is reported to yield 3 different isoforms for this protein. A member of the Pseudouridine synthase RluA protein family, it is known to catalyze uridine to pseudouridine isomerization (pseudouridylation) of specific mitochondrial mRNAs (mt-mRNAs), a post-transcriptional modification necessary for their translation. Other names for this target antigen include RNA pseudouridylate synthase domain containing 3, RNA pseudouridylate synthase domain-containing protein 3, and mitochondrial mRNA pseudouridine synthase RPUSD3. Gene orthologs have been identified in the mouse, rat, bovine, frog, chimpanzee and chicken species.