Anti-VPS53 subunit of GARP complex antibodies are used for the immunodetection of the protein encoded by the VPS53 gene. In humans, the canonical protein has a reported length of 832 amino acid residues and a mass of 94.4 kDa. Its subcellular localization is in the Golgi and membrane. Alternative splicing is reported to yield 4 different isoforms for this protein. A member of the VPS53 protein family, it is known to act as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The gene encoding this protein is implicated in Pontocerebellar hypoplasia 2E. Other names for this target antigen include PCH2E, hVps53L, pp13624, vacuolar protein sorting-associated protein 53 homolog, VPS53, GARP complex subunit, and HCCS1. Gene orthologs have been identified in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species.