Anti-NBAS subunit of NRZ tethering complex antibodies are used for the immunodetection of the protein encoded by the NBAS gene. In humans, the canonical protein has a reported length of 2371 amino acid residues and a mass of 268.6 kDa. Its subcellular localization is in the membrane, ER, and cytoplasm. Alternative splicing is reported to yield 2 different isoforms for this protein. It is widely expressed across many tissues, with highest levels in the heart and skeletal muscle, and lowest levels in the liver, small intestine and thymus. It is known to be involved in Golgi-to-endoplasmic reticulum (ER) retrograde transport; the function is proposed to depend on its association in the NRZ complex which is believed to play a role in SNARE assembly at the ER. Other names for this target antigen include NAG, NAG/BC035112 fusion, NAG/FAM49A fusion, neuroblastoma amplified sequence, and ILFS2. Gene orthologs have been identified in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species.