Anti-NOP2/Sun RNA methyltransferase 6 antibodies are used for the immunodetection of the protein encoded by the NSUN6 gene. In humans, the canonical protein has a reported length of 469 amino acid residues and a mass of 51.8 kDa. Its subcellular localization is in the cytoplasm. A member of the RsmB/NOP protein family, it is reported to be a S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 72 in tRNA(Thr)(TGT) and tRNA(Cys)(GCA). Other names for this target antigen include ARL5B antisense RNA 1, NOL1/NOP2/Sun and PUA domain-containing protein 1, NOL1/NOP2/Sun domain family, member 6, and tRNA (cytosine(72)-C(5))-methyltransferase NSUN6. Gene orthologs have been identified in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species.