Anti-SURF1 cytochrome c oxidase assembly factor antibodies are used for the immunodetection of the protein encoded by the SURF1 gene. In humans, the canonical protein has a reported length of 300 amino acid residues and a mass of 33.3 kDa. Its subcellular localization is in the mitochondria. Alternative splicing is reported to yield 2 different isoforms for this protein. A member of the SURF1 protein family, it is a known component of the MITRAC (mitochondrial translation regulation assembly intermediate of cytochrome c oxidase complex) complex, that regulates cytochrome c oxidase assembly. The gene encoding this protein is implicated in Mitochondrial complex IV deficiency. Other names for this target antigen include MC4DN1, SHY1, surfeit locus protein 1, surfeit 1, and CMT4K. Gene orthologs have been identified in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species.