Anti-COL5A3 antibodies are used in the immunodetection of the protein collagen type V alpha 3 chain. In humans, the canonical protein has a reported length of 1745 amino acid residues and a mass of 172.1 kDa. Its subcellular localization is in the extracellular matrix and is secreted. It is notably expressed in fibroblasts. A member of the Fibrillar collagen protein family, COL5A3 is known to be involved with cell matrix adhesion and extracellular matrix organization. Post-translational modifications have been described, including glycosylation.
The COL5A3 marker can be used to identify Cerebral Cortex Astrocytes and Hippocampal Gyrus Astrocytes.* Synonyms for this target antigen include collagen, type V, alpha 3, pro-(alpha)3(V) collagen, and collagen alpha-3(V) chain. COL5A3 gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish and chimpanzee species. A number of COL5A3 antibodies have been mentioned in research publications and have associated citations. Immunohistochemistry is a widely used application for these antibodies. ELISA, Western Blot, and Immunofluorescence are also common applications.
*HuBMAP Human Reference Atlas v1.4