Anti-FAM53B antibodies are used in the immunodetection of the protein family with sequence similarity 53 member B. In humans, the canonical protein has a reported length of 422 amino acid residues and a mass of 45.8 kDa. Its subcellular localization is in the nucleus. Alternative splicing is reported to yield 2 different isoforms for this protein. It is notably expressed in skeletal muscle, kidney, spleen, thyroid, testis, ovary, small intestine, colon and peripheral blood. A member of a the FAM53 protein family, FAM53B is known to act as a regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) nuclear localization. Synonyms for this target antigen include bA12J10.2, smp, protein FAM53B, protein simplet, simplet homolog, and KIAA0140.