Anti-hexosaminidase D antibodies are used for the immunodetection of the protein encoded by the HEXD gene. In humans, the canonical protein has a reported length of 486 amino acid residues and a mass of 53.8 kDa. Its subcellular localization is in the nucleus and cytoplasm. Alternative splicing is reported to yield 2 different isoforms for this protein. It is reported to be expressed in synovial fibroblasts and synovial membranes. A member of the Glycosyl hydrolase 20 protein family, it is known to have hexosaminidase activity. Other names for this target antigen include beta-N-acetylhexosaminidase, beta-hexosaminidase D, hexosaminidase (glycosyl hydrolase family 20, catalytic domain) containing, hexosaminidase D, cytosolic, and N-acetyl-beta-galactosaminidase. Gene orthologs have been identified in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. Western Blot is the most common application for the hexosaminidase D antibodies listed below. Immunofluorescence and Immunohistochemistry are also common applications.