Anti-CRB2 antibodies are used in the immunodetection of the protein crumbs cell polarity complex component 2. In humans, the canonical protein has a reported length of 1285 amino acid residues and a mass of 134.3 kDa. Its subcellular localization is in the cell membrane and cytoplasm. Up to 3 different isoforms have been reported for this protein. It is reported to be expressed in glomeruli, podocytes of the glomerular capillary loops, and parietal glomerular epithelial cells in the kidney. It is a member of the Crumbs protein family. Post-translational modifications have been described, including N-glycosylation.
The CRB2 marker can be used to identify Parietal Epithelial Cells.* Synonyms for this target antigen include crumbs 2, cell polarity complex component, crumbs family member 2, crumbs-like protein 2, and protein crumbs homolog 2. CRB2 gene orthologs have been reported in the mouse, rat, bovine, frog, chimpanzee and chicken species. ELISA, Western Blot, Immunofluorescence, and Immunohistochemistry are common applications for CRB2 antibodies.
*HuBMAP Human Reference Atlas v1.4