Anti-nuclear factor I A antibodies are used for the immunodetection of the protein encoded by the NFIA gene. In humans, the canonical protein has a reported length of 509 amino acid residues and a mass of 55.9 kDa. Its subcellular localization is in the nucleus. Alternative splicing is reported to yield 4 different isoforms for this protein. A member of the CTF/NF-I protein family, it is known to recognize and bind the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. The gene encoding this protein is implicated in Brain malformations with or without urinary tract defects. Other names for this target antigen include C1DELp32p31, CTF, DEL1P32P31, NF1-A, NFI-L, and BRMUTD. Gene orthologs have been identified in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. Over 60 citations in the literature describe the use of nuclear factor I A antibodies in research. Western Blot is a widely used application for these antibodies. In addition, Immunohistochemistry is also a common application.