Anti-RIF1 antibodies are used in the immunodetection of the protein replication timing regulatory factor 1. In humans, the canonical protein has a reported length of 2472 amino acid residues and a mass of 274.5 kDa. Its subcellular localization is in the nucleus and cytoplasm. Up to 2 different isoforms have been reported for this protein. It is reported to be highly expressed in the testis. A member of the RIF1 protein family, RIF1 is a reported key regulator of TP53BP1 that plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage: acts by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs. Synonyms for this target antigen include RAP1 interacting factor homolog, rap1-interacting factor 1 homolog, and telomere-associated protein RIF1. RIF1 gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. Over 70 citations in the literature describe the use of RIF1 antibodies in research. Western Blot is a widely used application for these antibodies. ELISA and Immunohistochemistry are also common applications.