Anti-Hemicentin 1 antibodies are used in the immunodetection of the protein encoded by the HMCN1 gene. In humans, the canonical protein has a reported length of 5635 amino acid residues and a mass of 613.4 kDa. Its subcellular localization is in the extracellular matrix, cytoplasm, and is also secreted. Alternative splicing is reported to yield 3 different isoforms for this protein. It is reported to be expressed in hair follicles and in the dermis. It is known to be involved in transforming growth factor beta-mediated rearrangement of the podocyte cytoskeleton which includes reduction of F-actin fibers and broadening, flattening and elongation of podocytes. Post-translational modifications have been described, including glycosylation. Other names for this target antigen include LOC100507250/HMCN1 fusion and fibulin-6.