Anti-DPH7 antibodies are used in the immunodetection of the protein diphthamide biosynthesis 7. In humans, the canonical protein has a reported length of 452 amino acid residues and a mass of 50.6 kDa. A member of a the DPH7 protein family, DPH7 is known to catalyze the demethylation of diphthine methyl ester to form diphthine, an intermediate diphthamide biosynthesis, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2) which can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A (Eta). Synonyms for this target antigen include diphthine methyltransferase and WD repeat-containing protein 85.