In 1987, Felgner and others first described the application of “Lipofection,” which used a synthetic reagent abbreviated as DOTMA to effectively deliver DNA into tissue culture cells. DOTMA comprised of cationic lipids that, once associated with negatively charged DNA, formed stable liposomal complexes. This gave rise to the now widely used method of synthetic liposomal-based transfection. Now, various commercial reagents have been thoroughly developed with features such as low toxicity, effectiveness on hard-to-transfect cells, as well as high reproducibility.