Invitrogen
RNA Later solution
AM7024
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I study gut immunobiology during early life, with a particular focus on how breastfeeding influences immune development and intestinal function. To preserve RNA integrity from tissues collected at experimental endpoints, I routinely use RNA later solution. This reagent is highly effective at stabilizing RNA and inhibiting RNase activity, which is particularly valuable for large-scale experiments involving the collection and processing of multiple tissue samples. Tissue samples can be stored in RNA later at 4°C for up to one week without significant loss of RNA quality, allowing flexibility in sample processing. Following preservation, RNA can be efficiently isolated for downstream gene expression analyses, including quantitative PCR (qPCR) and RNA sequencing (RNA-seq) applications
Very helpful in RNA isolation and downstream gene expression analysis
Any tissue isolates
Do not place the tissue directly into a –20°C or –80°C freezer. Store samples at 4°C overnight to allow the solution to penetrate. After overnight incubation, remove the excess RNA later solutoin and transfer to –20°C or –80°C for indefinite storage
The results shown here were generated from qPCR analysis of TGFβ1 (Tgfb1) transcript expression in tumor and non-tumor regions of the mouse colon collected at the experimental endpoint. Following tissue harvesting, samples were immediately preserved in RNA later solution and stored at 4°C for 48 hours to maintain RNA integrity. After the preservation period, total RNA was isolated from the tissues and used for downstream quantitative PCR (qPCR) analysis to assess Tgfb1 gene expression.
Cheap and save time.
Nothing bad about this.
Very easy to use, saves time, preserve RNA quality and better use. A must for RNA isolation and qPCR analysis from tissue.