Effective Dye for Visualizing Acidic Cellular Compartments

July 09, 2026

BlueskyReddit
MD Anderson Cancer Center - University of Texas
Cancer System Imaging (Diagnostic Imaging Division)
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Questions/Comments

Company:

Invitrogen

Product Name:

LysoSensor Blue DND-167

Catalog Number:

L7533

LysoSensor Blue DND-167 offers a convenient way to assess lysosomal acidification in live cells. The probe produced distinguishable staining patterns and responded appropriately to pH changes.

Experimental Design and Results Summary

Application

Used for lysosome tracking, autophagy investigations, intracellular trafficking studies, and pH-dependent cellular assays.

Starting Material

Live adherent or suspension cell cultures.

Tips

Run preliminary titration experiments to identify optimal staining conditions. Analyze samples promptly to preserve fluorescence quality.

Results Summary

The dye generated noticeable fluorescence within acidic vesicles and allowed detection of treatment-induced lysosomal changes. Signal quality was generally reproducible across experiments.

DOI or PMID #

N/A

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Summary

The Good

Quick staining procedure and straightforward imaging workflow. Suitable for monitoring dynamic lysosomal activity in living cells.

The Bad

Signal intensity may fluctuate depending on microscope settings and cell model. Careful optimization is often necessary for best performance.

The Bottom Line

A valuable probe for lysosomal research that delivers informative results when properly optimized.

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