Reliable Prestained Protein Marker for Routine WB

June 30, 2026

BlueskyReddit
University of Florida
Neurosurgery
Research Scientist

Overall

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Questions/Comments

Company:

GenDEPOT

Product Name:

Xpert 2 Prestained Protein Marker

Catalog Number:

P8503-025

I used the Xpert 2 Prestained Protein Marker as a molecular weight reference during SDS-PAGE analysis of protein samples. Loading 10 µL of the marker provided clear, well-resolved colored bands that made it easy to monitor electrophoresis and estimate protein molecular weights.

Experimental Design and Results Summary

Application

Protein molecular weight determination during SDS-PAGE and Western blot analysis.

Starting Material

Tips

Load 10 µL of the marker directly into one lane of the gel. The prestained bands are clearly visible during electrophoresis, making it easy to monitor the run and identify the molecular weight of protein bands.

Results Summary

Loading 10 µL of the marker produced sharp, well-resolved colored bands across the molecular weight range. The marker allowed straightforward monitoring of gel migration and accurate estimation of the sizes of experimental protein bands throughout SDS-PAGE and subsequent Western blot analysis.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Bright, clearly visible prestained bands

The Bad

N/A

The Bottom Line

The GenDEPOT Xpert 2 Prestained Protein Marker is an excellent choice for routine SDS-PAGE and Western blot experiments. Loading 10 µL provides clear, reproducible molecular weight standards that simplify electrophoresis monitoring and protein size estimation with minimal effort

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