Abcam
Anti-VDAC1/Porin + VDAC3 antibody
ab14734
Monoclonal; clone number: 20B12AF2
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In our study, we took a closer look at voltage-dependent anion channel 1 (VDAC1) expression in mouse brain tissue using immunostaining techniques. This approach served as an insightful indicator of mitochondrial content, allowing us to probe deeper into the fascinating world of cellular energy dynamics.
Immunofluorescence
Mouse 40-μm-thick coronal sections (perfused brain)
1:1000; Incubation at 4°C overnight
3% normal goat serum and 0.3% Triton X-100 in 0.01 M PBS
1:1000 (Alexa 568 anti-mouse fluorescent secondary antibody)
N/A
Images were collected with a Leica SP8 confocal microscope with PMT and HyD detectors.
We measured the expression of the mitochondrial marker VDAC1 in mouse prefrontal cortex astrocytes with deficient metabolism (lactate production and oxidative phosphorylation). An increase in VDAC1 can suggest increased mitochondrial content. In the image: left: red – GFAP (astrocyte marker); middle: cyan – VDAC1 (mitochondrial marker); right: merged image of GFAP and VDAC1.
For VDAC1 immunostaining, antigen retrieval was performed, followed by a standard immunostaining protocol.
Has good signal.
We used antigen retrieval for this antibody.
The antibody gives a strong signal and provides robust results throughout the experiments. Highly recommended.