Thermo Fisher Scientific mCherry Antibody Compatible with BaseScope

Results Summary

We used an adeno-associated virus, rAAV5/GFAP-GFP-Cre, to knock down the expression of Oprm1 in astrocytes of the hippocampus, a brain region particularly enriched with mu opioid receptors (Oprm1 encodes mu opioid receptors). We quantified the efficiency of knockdown with this AAV in hippocampal astrocytes by measuring Oprm1 mRNA transcripts in the transduced astrocytes. We used the mCherry antibody to visualize the transduced astrocytes.Image: Representative confocal image of dual BaseScope in situ hybridization for Oprm1 mRNA (red punctate dots) and GFAP (green), and mCherry (cyan) immunofluorescence. Immunostaining with an mCherry antibody was required, as BaseScope reagents quenched the viral fluorescence. Section was counterstained with DAPI fluorescent dye to visualize tissue morphology, specifically cell nuclei (blue). Image was obtained using a 63× objective; scale bar: 20 μm.