Sigma-Aldrich
Monoclonal Anti-PSD95 antibody produced in mouse
P246
7E3-1B8, monoclonal
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We utilize the PSD-95 antibody to evaluate the expression of the postsynaptic marker of glutamatergic synapses in the prefrontal cortex. We perform a single-cell, Imaris-based analysis of the spatial density of the postsynaptic marker of excitatory synapses and correlate it with mice's cognitive performance following different manipulations.
Immunofluorescence
Mouse samples; 40-μm thick coronal brain sections
1:1000; 4°C overnight
3% normal goat serum and 0.3% Triton X-100 in PBS for 1 h at room temperature
1:1000; Alexa 633-labeled anti-mouse fluorescent secondary antibody
N/A
Leica SP8 confocal microscope with PMT and HyD detectors
PSD-95 plays a crucial role in the stabilization of excitatory synapses. We measure PSD-95 together with VGLUT1 immunoreactivity to evaluate glutamatergic synaptic puncta. We found a significantly reduced density of VGLUT1⁺/PSD-95⁺ puncta in mice with deficient energy metabolism in astrocytes. Mice with altered energy metabolism in astrocytes and altered expression of postsynaptic markers of glutamatergic synapses within the territories of energy-deficient astrocytes showed cognitive deficits. Images: representative confocal images of brain samples (prefrontal cortex) from different mice.
Delivers sensitive, robust, and reproducible results.
Rather expensive for 100 µL.
Highly recommend, as it works as expected — it does not require any exhausting calibrations. The signal is very robust.