PE Anti-mouse Tim-3 Antibody With Specific Staining

February 23, 2024

Medicine
Weill Cornell Medicine
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-mouse CD366 (Tim-3) Antibody

Catalog Number:

119704

Clone Number:

RMT3-23

This antibody provides a good Tim-3 PE mouse staining on lymphocytes and an acceptable background. It allows good detection of mouse Tim-3-positive exhausted T cells.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse colonic cells

Primary Incubation

1/200 in PBS

Blocking Agent

Fc Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR Fortessa II

Results Summary

I use this antibody for detecting Tim-3-positive exhausted T cells among CD45-positive cells from mouse colon tissue. The staining was convincing and specific to perform proper gating strategy.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good and specific staining.

The Bad

Higher staining intensity could be achievable with proper antibody titration.

The Bottom Line

Good staining of Tim-3 in CD45-positive cells isolated from mouse colon tissues. Great for detecting exhausted T cells.

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