GFP Antibody From Cell Signaling Technology Produces Clear Bands

Houston Methodist Research Institute

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

GFP Antibody

Catalog Number:

2555S

Clone Number:

The performance of this antibody (GFP) in Western blots has been consistently reliable without any issues. Employing a 1:1000 dilution in BSA, particularly in HELA cells, has yielded satisfactory results. It's worth noting that the antibody has shown effectiveness in detecting the target under these conditions.

In addition, if you're working with GFP (Green Fluorescent Protein), considering the use of a GFP antibody could be beneficial for co-localization studies or assessing fusion protein expression. Ensure to optimize the dilution and experimental conditions for the GFP antibody to achieve optimal results in your specific experimental setup.

Experimental Design and Results Summary

Applications

Western blot

Sample

HELA cells

Primary Incubation

1:1000

Blocking Agent

5% BSA in TBST

Secondary Incubation

1:1000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

The performance of this antibody in Western blots has been consistently reliable without any issues. Employing a 1:1000 dilution in BSA, particularly in HELA cells, has yielded satisfactory results. It's worth noting that the antibody has shown effectiveness in detecting the target under these conditions.In addition, if you're working with GFP (Green Fluorescent Protein), considering the use of a GFP antibody could be beneficial for co-localization studies or assessing fusion protein expression. Ensure to optimize the dilution and experimental conditions for the GFP antibody to achieve optimal results in your specific experimental setup.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

The bands were clear.

The Bad

N/A

The Bottom Line

Highly recommended.

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