Reliable Live Dead Dye From BioLegend with Cell Fixation

January 22, 2024

University of Southampton
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Zombie Aqua™ Fixable Viability Kit

Catalog Number:

423101

Clone Number:

I used Zombie Viability dyes to examine dead cell frequency in flow cytometry samples. Samples needed to be fixed with formaldehyde so I used a live/dead dye which allows fixation.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

THP-1 cells

Primary Incubation

10min

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

Zombie Viability dyes allowed me examine dead cell frequency in tissue culture samples and exclude them from further analysis. This dye worked well with when used prior to formaldehyde fixation. Although extending the incubation beyond 10 min led to increased nonspecific pick up of dye. Titration was also useful to determine optimum signal to noise ratio.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Reliable staining.

The Bad

Titration is needed and provide with max incubation time.

The Bottom Line

A very reliable live dead dye to use before fixation of samples.

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