Great Nucleic Acid Quantification Kit From Thermo Fisher

Covaris
R&D
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

ThermoFisher Scientific

Product Name:

Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents

Catalog Number:

P7589

They were used to validate a DNA extraction workflow from various FFPE tissue types. DNA was extracted from 10 um scrolls cut from 10 different FFPE tissues.

Experimental Design and Results Summary

Application

Assessment of extraction quality

Starting Material

DNA from FFPE in Tris solution

Tips

You can save tips by pipetting the TE before your samples.

Results Summary

Results were nice, low CV, simple and quicker than qubit if you are running at least 16 samples. Pairs well with the ribogreen for RNA, same 96 well plate.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Optimal for 16 samples or more.

The Bad

Standard curve can be somewhat annoying to prepare.

The Bottom Line

Recommended for higher throughput but requires a plate reader and correct 96 well plates.

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