Robust GFP+ HUVECs for Visualizing Vascular Formation

December 27, 2023

University of Toronto
Chemical/Biomedical Engineering
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Angio-Proteomie

Product Name:

GFP Expressing Human Umbilical Vein Endothelial Cells

Catalog Number:

cAP-0001GFP

GFP HUVECs were grown in 3D in vitro tissues. Cell culture supplements and co-cultures were used to analyze the effects on vascular tube formation, visualized via fluorescent imaging of cells.

Experimental Design and Results Summary

Application

Modelling and visualizing vessel formation in vitro

Starting Material

HUVECs seeded in fibrin-based hydrogels

Tips

Adding supporting cells (such as pericytes) improves vessel formation

Results Summary

Organization of HUVECs into 3D vascular tubes can be enhanced in the presence of supporting cells as well as factors that stimulate a vasculogenic environment. It is easy to visualize vessel formation over time in 3D tissues using GFP+ HUVECs.

DOI or PMID #

N/A

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Summary

The Good

Easy to use out of the box compared to manually transfecting cells.

The Bad

Perhaps slightly different properties than regular HUVEC. GFP expression fades at later passages.

The Bottom Line

Great cells for fast and easy studies of vessel formation in vitro. Fluorescent imaging of GFP cells makes visualizing results simple and effective.

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