Brilliant Violet 605™ Anti-Human CD194 (CCR4) Antibody

January 03, 2024

JvGCRC
Nottingham Trent University
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 605™ anti-human CD194 (CCR4) Antibody

Catalog Number:

359418

Clone Number:

L291H4

This antibody has been used to optimize flow cytometry-based immunophenotyping of PBMC samples. The results have not yet been published. Multi-colour antibody panels were designed to identify several lymphocyte populations and tested to ensure appropriate spectral unmixing was possible in a 20+ color panel. This antibody was used to help identify subpopulations of T helper cells.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Human PBMC

Primary Incubation

1.25µL Brilliant Violet 605™ CD194 (CCR4) for 30 minutes at 4°C

Blocking Agent

Human TruStain FcX™ (Biolegend 422302) and CellBlox™ Plus (Thermo Fisher C001T06F01)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Acquired on 3L Cytek Aurora, analysed using Kaluza Software

Results Summary

Clear distinction between positive and negative populations. This antibody helped identify subpopulations of T helper cells in a large multi-colour staining panel for spectral cytometry. Staining was clear even after significant titration from manufacturers suggested volume.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Able to titrate and still retain clear, distinct staining.

The Bad

Used in a large panel so extra care had to be taken to avoid overlap with other fluorophores.

The Bottom Line

Very good antibody that fit well into a large multi-colour panel.

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