I Achieved Specific Staining Using This APC Anti-Mouse KLRG1 Antibody

January 03, 2024

Medicine
Weill Cornell Medicine
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC anti-mouse/human KLRG1 Antibody

Catalog Number:

138412

Clone Number:

2F1/KLRG1

This antibody provides a good KLRG1 mouse staining on T cells and an acceptable background. It allows good detection of mouse KLRG1-positive T cells.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Tumor-infiltrating immune cells from MC38 subcutaneous tumor

Primary Incubation

1/200 in PBS

Blocking Agent

Fc Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR Fortessa II

Results Summary

I use this antibody for detecting KLRG1-positive T cells from tumor-infiltrating mouse subcutaneous MC38 tumor tissue. The staining was convincing and specific to perform proper gating strategy.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good and specific staining.

The Bad

Require antibody titration to improve the staining at the best.

The Bottom Line

Good staining of KLRG1 in mouse tumor-infiltrating lymphocytes in a subcutaneous MC38 model. Great for detecting KLRG1 differentiated T cells.

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