Excellent PE Anti-Mouse B220 Antibody From Thermo Fisher

December 29, 2023

Medicine
Weill Cornell Medicine
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher

Product Name:

CD45R (B220) Monoclonal Antibody, PE

Catalog Number:

12-0452-82

Clone Number:

RA3-6B2

This antibody provides a good CD44 mouse staining on lymphocytes and an acceptable background. It allows good detection of mouse B cells.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Tumor-infiltrating immune cells from MC38 subcutaneous tumor

Primary Incubation

1/200

Blocking Agent

Fc Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR Fortessa II

Results Summary

I use this antibody for detecting B220-positive B cells from tumor-infiltrating mouse subcutaneous MC38 tumor tissue. The staining was convincing and specific to perform proper gating strategy.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good and specific staining.

The Bad

Staining intensity is good but could be stronger with a PE fluorochrome.

The Bottom Line

Good staining of B220 in mouse tumor-infiltrating lymphocytes in a subcutaneous MC38 model. Great for detecting B cells in tissues.

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