Invitrogen
DAPI (4',6-Diamidino-2-Phenylindole, Dilactate)
D3571
Info:Supplier Page
DAPI is a water-soluble dye and emits blue fluorescence upon binding to AT regions of DNA which makes it a popular nuclear counterstain. Also, DAPI is a cell non-permeant dye, and I used it to counterstain the nucleus in virus-infected cells after fixation of cells with formaldehyde.
Cell Imaging
Human lung cell line
Stain the targeted cells after fixation as it is cell non-permeable dye.
Clear bright blue color nucleus was imaged in fluorescence microscopy in both virus infected and non-infected cells.
N/A
Require very less time to counterstain the cells.
None.
Great signal and less time consuming.