Good Anti-KLRG1 Antibody From BioLegend for the Flow Cytometric Detection of Effector T Cells

October 20, 2023

Department of Immunology and Microbiology
Scripps Research
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

Brilliant Violet 711™ anti-mouse/human KLRG1 (MAFA) Antibody

Catalog Number:

138427

Clone Number:

2F1/KLRG1

The BV 711 KLRG1 antibody can be used for identifying effector CD8+ T cells within immune cell populations isolated from mouse lungs, spleen, and lymph nodes. The antibody has given good separation at dilution of 1:200 and BV711 typically does not have too many compensation issues. This antibody is known to have very poor affinity, so I am not surprised by the high concentration needed for effective staining.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse lung, mediastinal lymphnode, spleens

Primary Incubation

1:200, for 30 mins in dark at 4 degrees C

Blocking Agent

Fc Shield

Secondary Incubation

BD cytofix/cytoperm; 100 ul for 20 mins at 4C

Tertiary Incubation

None

Detection

5 laser Aurora Flow cytometer

Results Summary

I used this product to identify effector CD8+ T cells in mice immunized with antigen. Cells were isolated from lungs, Spleen through digestion or smashing through 70 um filters; RBC lysis was performed using ACK buffer. For lungs, Percoll was used for enrichment of lymphocytes. Cells were then stained with viability dye followed by extracellular antibodies such as TCR beta, CD4, CD8, CD127, KLRG1, CD69, CD103.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specific, bright, and versatile for numerous panels.

The Bad

Poor affinity for antigen.

The Bottom Line

BV 711 conjugated KLRG1 worked well for my panel to identify tetramer specific effector memory CD8+ T cells. I would highly recommend this antibody fluorophore combination for flow cytometry.

Share your experience with other scientists. Write a Review! »

Join the discussion