Native Gels for Separating Mitochondrial Respiratory Complexes

Stanford University
Department of Pathology
Postdoctoral Researcher

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Company:

ThermoFisher Scientific

Product Name:

NativePAGE™ 3 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gels

Catalog Number:

BN1001BOX

Info: Supplier Page 

The native gels were used to separate and study the dynamic organization of mitochondrial respiratory complexes under different conditions.

Experimental Design and Results Summary

Application

Gel electrophoresis

Starting Material

Mitochondrial fraction, Isolated mitochondria

Tips

Choose the right percentage gradient gel according to the size of the complex to study.

Results Summary

Native gel stained with Coomassie Brilliant Blue showing separation of mitochondrial protein complexes. Dots indicate the respiratory complexes identified in the indicated slices.

DOI or PMID #

doi:10.1242/jcs.248492

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Summary

The Good

Ease of use, better separation, and does not break on handling.

The Bad

Sample volume limitation, and expensive.

The Bottom Line

Easy to use and good quality native gels for reproducible protein complex separation.

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