Proteintech
Anti Mouse GAPDH Monoclonal antibody
60004-1-Ig
N/A
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My research involves studying how D1 dopamine receptor, a member of the GPCR superfamily, can signal from distinct membrane compartments within the cell. I have found that D1 dopamine receptor can be activated at the plasma membrane and from endosomes after internalization from the cell surface. I have also found that this receptor exists as a stable pool at the Golgi apparatus and can signal from there as well.
Western Blot
HeLa and HEK293 cell lysates
1:5000
3% milk in TBS-Tween
1:10000, 1hr, RT on shaker
Chemiluminescent substrates, 2min RT
ECL then film/image dock
This product helped provide a loading control for our Western Blot experiments to ensure that the same amount of general protein content was added to each well.
Worked very well, no optimization required.
Nothing bad to report.
Great signal, decent price.