Wonderful Peripherin Antibody from Abcam

Afyon Kocatepe University

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Recombinant Anti-Peripherin antibody

Catalog Number:

ab246502

Clone Number:

EPR23445-28

It is very specific and pure rabbit monoclonal antibody. You can get strong signal using standard antigen retrieval method (citrate pH 6.0) with 1/1000 dilution rate.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Rat intestine

Primary Incubation

1/1000, RT, 2 hours

Blocking Agent

BSA, 1%, 10'

Secondary Incubation

Anti-rabbit IgG, 1/200 dilution, RT.

Tertiary Incubation

Peroxidase-strepteavidin complex (1 hours at RT) and tehn AEC chromogen, RT 20'

Detection

ABC-peroxidase technique

Results Summary

As seen in the photograph, it binds to Auerbach's plexus and intermuscular nerves with high affinity. It is very clean staining. There is no background staining and nonspecific reaction.

DOI or PMID #

N/A

Additional Notes

Gill's hemalum as a background staining.

Image Gallery

Summary

The Good

Strong and specific signal with high dilution rate.

The Bad

Nope

The Bottom Line

It is an antibody that I highly recommend it. Strong, specific and high dilution rate.

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