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Alexa Fluor® 488 anti-mouse CD69 Antibody
104516
H1.2F3
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) in health and disease. In this experiment we wanted to have a anti-CD69 that works. We used T cells as control.
Flow Cytometry
Activated mouse splenocytes
Surface staining with CD69 A488 (1/100), 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometry
CD69 is rapidly upregulated upon stimulation of T cells. Thus, we incubated splenocytes with plate bound anti-CD3, anti-CD28 or not for 30 min. On the figure, on the left there is the expression of CD69 of control cells, incubated with cRPMI only, while on the right there are the cells that were incubated with anti-CD3, anti-CD28, which up regulate CD69.
It works.
The signal is not very bright.
This antibody can be used.