Antibody From BioLegend Stains CD138 Very Well

Stanford

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 650™ anti-mouse CD138 (Syndecan-1) Antibody

Catalog Number:

142518

Clone Number:

281-2

Our lab works in the field of cancer, and we treated mice with immunotherapy. We wanted to assess how the immunotherapy in tumor-bearing mice would affect B cells in the spleen, so we used the antibody to identify CD138+ B cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse spleen.

Primary Incubation

1:100

Blocking Agent

FcBlock

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Attune NxT cytometer.

Results Summary

We stained mouse spleen cells with the anti-CD138 antibody and found that it identifies CD138+ B cells very well. The antibody was easy to use and the staining was very clear.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

The antibody works great for staining CD138.

The Bad

None to mention.

The Bottom Line

The antibody identifies CD138+ B cells very clearly.

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