Great HLA-DR Antibody for Immunohistochemistry

Afyon Kocatepe University

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Quality of Results

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Company:

Santa Cruz Biotechnology

Product Name:

HLA-DR

Catalog Number:

sc-53319

Clone Number:

TAL 1B5

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I use it in the differential diagnosis of some cat and dog tumors. A sharp and clean staining is obtained at high titer. The antibody that also works in rats and mice tissues.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Canine skin tissue

Primary Incubation

1/200

Blocking Agent

%1 BSA

Secondary Incubation

Biotinylated anti-mouse IgG

Tertiary Incubation

Peroxidase conjugated streptavidin - AEC chromogen

Detection

ABC-peroxidase method

Results Summary

A highly effective marker in the detection of leukocytic tumors. I use it to differentiate tumors with round morphology from other epithelial or mesenchymal origins.

DOI or PMID #

N/A

Additional Notes

Gill's hemalum as a background staining.

Image Gallery

Summary

The Good

Specific binding and without background staining.

The Bad

Nothing bad.

The Bottom Line

A highly effective marker in the detection of leukocytic tumors. I use it to differentiate tumors with round morphology from other epithelial or mesenchymal origins.

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